Gambaran histologi ovarium sapi aceh pascavitrifikasi menggunakan dimetyl sulfoksida dengan konsentrasi berbeda
This study aims to determine the morphology of Aceh bovine ovarian pasca vitrification, after its been exposed to dimetyl sulfoksida (DMSO) cryoprotectant. This study use a completely randomized design of one-way pattern ANOVA acquire three replications. Ovaries used in this study are Aceh cattle ovarian which collected from slaughter house (RPH) accounted as 4 organs, to the next sliced into 9 pieces. Ovarian pieces are then grouped into 3 treatment groups namely ovaries which exposed into solution containing DMSO 10% (P1), 20% (P2), and 30% (P3). The results showed that the average number of normal follicles is highest at P1 41,33 ± 32,51; followed by P2 20,00 ± 16,09 , and P3 15,66 ± 10,50. It was concluded that the ovarian tissue was exposed with DMSO 10% was able to maintain of the normal follicle than DMSO 20% and 30% although statistical test results showed no significant difference between group (P>0,05).
Djuwita I, Boediono A, Rusiyantono Y, Mohamad K, Herliatien. 2000. Perkembangan oosit kambing setelah maturasi, fertilisasi dan kultur in vitro. Media Veteriner. 7(4):11-17.
Donnez J, Martinez-Madrid B, Jadoul P, Van Langendonckt A, Demylle D. 2006. Ovarian tissue cryopreservation and transplantation: a review. Hum. Reprod. Update. 12(5):519-535.
Fukui EJ, Xia L, Downey BR. 1993. Ultrastructural changes in bovine oocyte cryopreserved by vitrification. Cryobiology. 32(2):139-156.
Kasai M. 2002. Advances in the cryopreservation of mammalian oocytes and embryos: development of ultra rapid vitrification. Rev. 1:1-9.
Lucci CM, Scheier LL, Machado GM, Amorim CA, Bao SN. 2007. Effect of storing pig ovaries at 4 or 20°C for different periods of time on the morphology and viability of pre-antra follicles. Reprod. Dom. Anim. 42:76-82.
Newton H, Fisher J, Arnold JR, Pegg DE, Faddy MJ, Gosden RG. 1998. Permeation of human ovarian tissue with cryoprotective agents in preparation for cryopreservation. Hum. Reprod. 13:376-380.
Pamungkas FA. 2010. Pemanfaatn metode vitrifikasi untuk kriopreservasi oosit mamalia. Wartazoa. 20(3):112-116.
Shaw JM, Oranratnachai A, Trounson AO. 2000. Fundamental cryobiology of mammalian oocytes and ovarian tissue. Theriogenology. 53:59-72.
Steel RGD, Torrie JH. 1993. Prinsip dan Prosedur Statistik. (Diterjemahkan oleh: Soemantri). Gramedia: Jakarta.
Vieira AD, Mezzalira A, Barbieri DP, Lehmkuhl RC, Rubin MIB, Vajta G. 2002. Calves born after open pulled straw vitrification of immature bovine oocytes. Cryobiology. 45:91-94.
Wahjuningsih S, Hardjopranjoto S, Sumitro SM. 2010. Pengaruh konsentrasi etilen glikol dan lama paparan terhadap tingkat fertilitas in vitro oosit sapi. Jurnal Kedokteran Hewan. 4(2):61-64.
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