Optimasi Penggunaan Mutagen Kolkisin untuk Peningkatan Produktivitas Tanaman Stylo (Stylosanthes guianensis (Aubl.) Sw.)

Optimalization of Colchicine Mutagen Utilization to Increase Stylo Productivity (Stylosanthes guianensis (Aubl.) Sw.)

  • N Yulia Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, Institut Pertanian Bogor
  • I Prihantoro Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, Institut Pertanian Bogor
  • P D M H Karti Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, Institut Pertanian Bogor
Keywords: colchicine level, productivity, soaking time, stylo

Abstract

Colchicine is a chemical that induced mutation in plants through doubling of plant chromosomes so that polyploid plants are produced which have a larger size than diploid plants. This study aimed to determine the optimal level and soaking time of colchicine to increase the productivity of stylo plants. This study used a Completely Randomized Factorial Design (CRD) with two factors and six replications. Factor A is a the colchicine level of 0 ppm, 25 ppm, 50 ppm, 75 ppm and 100 ppm. Factor B is the soaking time of 12 hours, 24 hours and 36 hours. The variables observed were vertical height, stem diameter, leaflets length, leaflets width and number of trifoliate leaves. The results showed that the colchicine at a level of 25 ppm with soaking time of 12 hours had a significant effect (p<0.05)   on the increase in plant vertical height, stem diameter, and leaf width by 75.62 cm, 3.08 mm and 0.90 cm, respectively.  The number of trifoliate leaves from the colchicine treatment and the length of the leaflets were not significantly different. The conclusion of this research is colchicine level of 25 ppm and soaking time of 12 hours can increase plant vertical height, stem diameter and leaf width on stylo plants aged 14 WAP (Weeks After Planting).

Key words:        colchicine level, productivity, soaking time, stylo

Downloads

Download data is not yet available.

References

Adisewoyo SS. 1995. Sitogenetika. Yogyakarta (ID): Gajah Mada University Press.

Aili EN, Respartijati & Sugiharto AN. 2016. Pengaruh pemberian kolkisin terhadap penampilan fenotip galur inbrida jagung pakan (Zea mays L.) pada fase pertumbuhan vegetatif. Jurnal Produksi Tumbuhan. 4(5):370-377.

As’adah M, Rahayu T & Hayati A. 2016. Metode pemberian kolkisin terhadap respon morfologi tanaman zaitun. Jurnal Biosaintropis. 2(1):46-52.

Crowder LV. 1986. Genetika Tumbuhan. Yogyakarta (ID): Gajah Mada University Press.

Gultom T. 2016. Pengaruh pemberian kolkisin terhadap jumlah kromosom bawang putih (Allium sativum) lokal kultivar doulu. Journal of Biosciences. 2(3):165-172.

Hayati A, Saraswati DR & Rahayu T. 2017. Kajian pemberian kolkisin dengan metode tetes terhadap profil poliploidi tanaman zaitun (Olea europaea). E-Jurnal Ilmiah Biosaintropis. 2(2):24-29.

Jain SM & Gupta SD. 2013. Biotechnology of Neglected and Underutilized Crops. New York (US): Spinger.

Litbang Pertanian. 2012. Tanaman Stylo (Stylosanthes guianensis) sebagai Pakan Ternak Ruminansia. Agroinovasi Edisi 22-28. Jakarta (ID): Litbang Pertanian.

Mansyurdin. 2000. Penggandaan Kromosom Tanaman Cabai Keriting dan Cabai Rawit. Padang (ID): Universitas Andalas Press.

Murni D. 2010. Pengaruh perlakuan kolkisin terhadap jumlah kromosom dan fenotip tanaman cabe kering (Capsicum annuum L.). Jurnal Agroekoteknologi. 2(1):43-48.

Nuraida D. 2012. Pemuliaan tanaman cepat dan tepat melalui pendekatan marka molekuler. El-Hayah. 2(2):97-103.

Pemerintah Indonesia. 2005. Peraturan Pemerintah Republik Indonesia Nomor 21 Tahun 2005 tentang Keamanan Hayati Produk Rekayasa Genetik. Jakarta (ID): Kementerian Keuangan Republik Indonesia.

Rahayu EMD, Sukma D, Syukur M, Aziz SA & Irawati. 2015. Induksi poliploid menggunakan kolkisin secara in vivo pada bibit anggrek bulan (Phalaenopsis amabilis (L.)). Buletin Kebun Raya. 18(1):41-48.

Ranney G. 2002. Poliploidy: From Evolution to Landscape Plant Improvement. Raleigh (US): North Carolina State University.

Sinaga E, Bayu E & Hasyim H. 2014. Pengaruh konsentrasi kolkisin terhadap pertumbuhan dan produksi kacang hijau (Vigna radiate L.) Jurnal Agroekoteknologi USU. 2(3):1238-1244.

Su XA, Dion V, Gasser SM & Freudenreich CH. 2015. Regulation of recombination at yeast nuclear pores controls repair and triplet repeat stability. Genes and Development. 29(1):1006-1017.

Sulistianingsih R, Suyanto ZA & Noer AE. 2004. Peningkatan kualitas anggrek dendrobium hibrida dengan pemberian kolkisin. Journal Agriculture and Environment. 11(1):13-21.

Suminah, Sutarno & Setyawan AD. 2002. Induksi poliploid bawang merah (Allium ascalonicum L.) dengan pemberian kolkisin. Jurnal Biodiversitas. 3(1):174-180.

Valentino, Nasir B, Toana MH. 2020. Pengaruh ekstrak akar tuba (Derris elliptica Benth). terhadap mortalitas Pomacea Canaliculata Lamarck. (mesogastropoda: ampullariidae) pada padi Oryza sativa L. Jurnal Agroland. 27(1):89-98.

Wiendra NMS, Pharmawati M & Astiti NPA. 2011. Pemberian kolkhisin dengan lama perendaman berbeda pada induksi poliploidi tanaman pacar air (Impatiens balsamina L.). Jurnal Biologi. 15(1): 9-14.

Wu FH, Yu XD, Zhuang NS, Liu GD & Liu JP. 2015. Induction and identification of Stylosanthes guianensis tetraploids. Genetic Molecular Research. 14(4):12692-12698

Published
2022-04-20
How to Cite
YuliaN., PrihantoroI., & KartiP. D. M. H. (2022). Optimasi Penggunaan Mutagen Kolkisin untuk Peningkatan Produktivitas Tanaman Stylo (Stylosanthes guianensis (Aubl.) Sw.): Optimalization of Colchicine Mutagen Utilization to Increase Stylo Productivity (Stylosanthes guianensis (Aubl.) Sw.). Jurnal Ilmu Nutrisi Dan Teknologi Pakan, 20(1), 19-24. https://doi.org/10.29244/jintp.20.1.19-24