Studi Pendahuluan Metode Biohidrolisis Kulit Buah Durian untuk Pembentukan Gula Reduksi dalam Produksi Bioetanol

Abstract

Biohydrolysis is an enzymatic process using microorganism as biological agent for lignoselulose biomass hydrolysis/delignification. Isolate collection of fungi Trametes polyzona and Aspergillus sp. has an ability as ligninolytic fungi for hydrolysis process on bioethanol production. This study analyzed the potential fungi isolate for biohydrolysis method for reducing sugar determination on durian rind. Optimization of biohydrolysis method were conducted by treatments namely direct biohydrolysis, combination of chemical pretreatment (1% NaOH) and biohydrolysis, the combination of heat pretreatment (microwave) and biohydrolysis, and alkali delignification using 5% NaOH as control. Fungi isolate of Trametes polyzona showed a better result as the best potential ligninolytic fungi than Aspergillus sp. Biohydrolysis process with Trametes polyzona both on 10 minutes heat pretreatment combination and direct biohydrolysis methods within 7 days of incubation resulted the best available reducing sugar on hyrolysate were 0.38% and 0.32% respectively. The direct hydrolysis method with Trametes polyzona result was not significantly different than control (0.32%). Both method of these biohydrolysis optimization can be conducted as an alternative for bioethanol production on durian rind. Trametes polyzona biohydrolisis method needs a further experiments with other influence parameters to get an effective method for more available reducing sugar on durian rind for bioetanol production.

Keywords: delignification, lignocellulose, optimazitation, Trametes polyzona