Keragaman Gen BG1 sebagai Kandidat Gen Penciri Ketahanan Penyakit pada Calon Galur Ayam IPB-D2

N. N. Miraj; C. Sumantri; S. Murtini; N. Ulupi

doi:10.29244/jipthp.10.3.144-151

N. N. Miraj Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor
C. Sumantri Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor
S. Murtini Departemen Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
N. Ulupi Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor

DOI: https://doi.org/10.29244/jipthp.10.3.144-151

Abstract

The BG1 gene is a disease resistance candidate gene in chicken, which is located in the MHC region. The mechanism of the BG1 gene is known as an inhibitor of T lymphocyte cell activation. This study aims to identify the diversity of the SNP gene BG1 in the IPB-D2 chicken line. IPB-D2 chicken is a candidate for a new line from a cross from IPB-D1 chicken, which was selected for high body resistance and good productivity. A total of 27 DNA samples of IPB-D2 (G0) chickens and 58 samples of IPB-D2 (G2) chickens were sequenced to identify the diversity of BG1 genes. The study found 6 point mutations (g.4489 G>T, g.4712 C>T, g.4741 T>C, g.4747 T>C, g.4749 T>C, g.4761 G>A) and deletion of 2 nucleotide bases g.4758 - g.4759 delAT. The combination of 6 SNPs forms 4 haplotypes. The SNPs that changed the amino acid composition were SNP g.4489 G>T cys> phen, g.4712 C>T gln>codon stop, g.4749 T>C val>ala and g.4761 G> A gly> glu. All mutations are classified as polymorphic, low heterozygosity value, and not in Hardy-Weinberg equilibrium.

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