Infeksi Alami Pepper Yellow Leaf Curl Virus dan Sweet potato virus C Pada Ubi Jalar di Malang, Jawa Timur

  • Tri Asmira Damayanti Departemen Proteksi Tanaman, Fakultas Pertanian, IPB
  • Anastasya Hondo IPB University
  • Yusmani Prayogo Balai Penelitian Aneka Kacang dan Umbi, Malang
Keywords: cylindrical inclusion, double infection, geminivirus, sweet potato

Abstract

Virus-like infection with vein yellowing and leaf malformation symptoms were observed on sweet potato IR Melati at Kendalpayak, Malang, East Java. Amplification of DNA/cDNA was carried out using universal primer of Begomovirus, Potyvirus and Cucumovirus showed successfully amplified by universal primer of Begomovirus and Potyvirus, while negatively to universal primer of Cucumovirus. Based on nucleotide sequences, the vein yellowing and leaf malformation symptoms on sweet potato caused by double infection of Pepper yellow leaf curl virus (PYLCV) and Sweet potato virus C (SPVC). DNA identity analysis using BioEdit software showed highest homology up to 98.5% to one of PYLCV isolates from Bali, and up to 98.0% to sweet potato isolate of SPVC from Japan and USA. This is a new finding of natural infection of PYLCV and SPVC on sweet potato in Indonesia.

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References

Albuquerque LCD, Inoue-Nagata AK, Pinheiro B, Ribeiro S, Resende RO, Moriones E, Navas-Castillo J. 2011. A novel of monopartite Begomovirus infecting sweetpotato in Brazil. Arch Virol. 156(7):1291–1294. DOI: https://doi.org/10.1007/s00705-011-1016-x.

Anjarsari L, Suastika G, Damayanti TA. 2013. Deteksi dan identifikasi Potyvirus pada ubi jalar di Tanah Toraja, Sulawesi Selatan. J Fitopatol Indones. 9(6):193–201. DOI: https://doi.org/10.14692/jfi.9.6.193.

Aramburu J, Galipienso L, Lopez C. 2007. Reappearance of Cucumber mosaic virus isolates belonging to subgroup IB in Tomato Plants in North-Eastern Spain. J Phytopathol. 15(5):513–518. DOI: https://doi.org/10.1111/j.1439-0434.2007.01267.x.

[Balitkabi] Balai Penelitian Tanaman Aneka Kacang dan Umbi. 2014. Penyakit virus tanaman ubi jalar dan upaya pengendaliannya Indonesia. Malang (ID): Balitkabi.

[BPS] Badan Pusat Statistik Provinsi Jawa Barat. 2016. Potensi ubi jalar di Jawa Barat [Internet]. [diunduh: 2017 Okt 2]. Tersedia pada: http://regional investment.bkpm.go.id/newsipid/id/commodityarea.php?ia=3201&ic=260

Doyle JJ, Doyle JL. 1987. A rapid DNA isolation of procedure for small quantities of fresh leaf tissue. Phytochem Bull. 19:11–19.

Gutierrez DL, Fuentes S, Salazar FL. 2003. Sweet potato virus disease (SPVD): distribution, incidence, and effect on sweet potato yield in Peru. Plant Dis 87:297–302. DOI: https://doi.org/10.1094/PDIS.2003.87.3.297.

Ha C, Coombs S, Revill PA, Harding RM, Vu M, Dale JL. 2008. Design and application of two novel degenerate primer pairs for the detection and complete genomic characterization of Potyviruses. Arch Virol. 53(1):25–36. DOI: https://doi.org/10.1007/s00705-007-1053-7.

Hondo A, Damaiyanti KC, Hafizh MF, Amanatillah N, Damayanti TA. 2018. Penyakit bercak kuning ubi jalar di Bogor, Jawa Barat. J Fitopatol Indones. 14(2):69–73. DOI: https://doi.org/10.14692/jfi.14.2.69.

Hull R. 2014. Plant Virology. Fifth Edition. London (UK): Academic Press. DOI: https://doi.org/10.1016/C2010-0-64974-1.

Kokkinos CD, Clark CA. 2006. Real-time PCR assays for detection and quantification of sweet potato viruses. Plant Dis. 9(6):783–788. DOI: https://doi.org/10.1094/PD-90-0783.

Kon T, Kuwabara K, Hidayat SH, Ikegami M. 2007. A Begomovirus associated with ageratum yellow vein disease in Indonesia: evidence for natural recombination between tomato yellow leaf curl Java virus and Ageratum yellow vein virus-[Java]. Arch Virol. 52(6):1147–1157. DOI: https://doi.org/10.1007/s00705-006-0928-3.

Li R, Salih S, Hurtt S. 2004. Detection of begomoviruses in sweetpotato by polymerase chain reaction. Plant Dis. 88(12):1348–1351. DOI: https://doi.org/10.1094/PDIS.2004.88.12.1347.

Lotrakul P, Valverde RA, Clark CA, Sim J, Torre RD. 1998. Detection of a Begomovirus infecting sweet potato in the United States. Plant Dis. 82(11):1253–1257. DOI: https://doi.org/10.1094/PDIS.1998.82.11.1253.

Mahendra IBG, Phabiola TA, Yuliadhi KA. 2017. Pengaruh infeksi beberapa virus terhadap penurunan hasil produksi tanaman tomat (Solanum lycopersicum Mill.) di Dusun Marga Tengah, Desa Kerta, Kecamatan Payangan, Kabupaten Gianyar. Agrotek Trop. 6(3):301–309.

Miano DW, LaBonte DR, Clark CA, Valverde RA, Hoy MW, Hurtt S, Li R. 2006. First report of a Begomovirus infecting Sweet potato in Kenya. Plant Dis. 90(6):832. DOI: https://doi.org/10.1094/PD-90-0832B.

Pusdatin Pertanian. 2016. Outlook komoditas pertanian sub sektor tanaman pangan. Jakarta (ID): Pusat Data dan Informasi Pertanian, Kementan.

Rahim YF, Damayanti TA, Ghulamahdi M. 2015. Deteksi virus yang menginfeksi kedelai di Jawa. J Fitopatol Indones. 11(2):59–67. DOI: https://doi.org/10.14692/jfi.11.2.59.

Sulandari S, Suseno R, Hidayat SH, Harjosudarmo J, Sosromarsono S. 2006. Deteksi dan kajian kisaran inang virus penyebab penyakit daun keriting kuning cabai. Hayati 13(1):1–6. DOI: https://doi.org/10.1016/S1978-3019(16)30371-0.

Trisno J, Hidayat SH, Jamsari, Habazar T, Manti I. 2010. Identifikasi molekuler Begomovirus penyebab penyakit kuning keriting pada tanaman cabai (Capsicum annum L.) di Sumatera Barat. J Natur Indones. 13(1):41–46. DOI: https://doi.org/10.31258/jnat.13.1.41-46.

Valverde RA, Clark CA, Valkonen JP. 2007. Viruses and virus disease complexes of sweet potato. Plant Viruses 1(1):116–126.

Published
2019-12-11
How to Cite
Damayanti, T. A., Hondo, A., & Prayogo, Y. (2019). Infeksi Alami Pepper Yellow Leaf Curl Virus dan Sweet potato virus C Pada Ubi Jalar di Malang, Jawa Timur. Jurnal Fitopatologi Indonesia, 15(6), 248-254. https://doi.org/10.14692/jfi.15.6.248-254
Section
Disease Note