DNA BARCODING UNTUK VALIDASI SPESIES PAUS SPERMA (Physeter macrocephalus LINNAEUS, 1758) DARI PERAIRAN LAUT SAWU, NUSA TENGGARA TIMUR, INDONESIA

  • Agus Alim Hakim Departemen Manajemen Sumberdaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor.
  • Endah Sri Rahayu Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, IPB University, 16680, Indonesia
  • Ali Mashar Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, IPB University, 16680, Indonesia
  • Nurlisa Alias Butet Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, IPB University, 16680, Indonesia
  • Yusli Wardiatno Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, IPB University, 16680, Indonesia
  • Mohammad Mukhlis Kamal Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, IPB University, 16680, Indonesia
DOI: https://doi.org/10.29244/coj.v7i1.49702
Kata Kunci: COI, DNA barcoding, genetik, Laut Sawu, paus sperma

Abstrak

Paus sperma (Physeter macrocephalus) merupakan salah satu biota laut yang dilindungi di Indonesia. Identifikasi molekuler paus sperma dari perairan Laut Sawu (Nusa Tenggara Timur, Indonesia) telah dilakukan menggunakan gen 16S rRNA. Penelitian ini bertujuan untuk menvalidasi spesies paus sperma (Physeter macrocephalus) di Indonesia menggunakan gen cytochrome oxidasesubunit I (COI) parsial. Spesimen yang digunakan merupakan spesimen jaringan dari koleksi laboratorium yang diambil dari perairan Laut Sawu tahun 2014. Ketiga spesimen tervalidasi sebagai Physeter microcephalus dengan kemiripan sebesar 100%. Komposisi basa didominasi oleh basa timin (31,7-31,8%) dengan GC konten sebesar 42,7–42,8%. Jarak genetik pada spesies yang sama didapatkan nilai sebesar 0,0015, sedangkan jarak genetik tertinggi dengan spesies Orcaella brevirostris sebesar 0,2128. Identifikasi molekuler menggunakan gen 16S rRNA dan COI telah menvalidasi spesies paus sperma (Physeter macrocephalus) di Indonesia, sehingga data genetik ini dapat digunakan sebagai dasar pengelolaan dan konservasi sumberdaya paus sperma. 

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Diterbitkan
2023-10-24
Terbitan
Vol 7 No 1 (2023): COJ (Coastal and Ocean Journal)
Bagian
Articles