Variation and Association of Avian β-Defensin 2 Gene with the Concentration of Immunoglobulin Y and the Titer of Newcastle-Disease Antibody in IPB-D1 Chicken

D. Lestari, Masruroh, I. Khaerunnisa, S. Murtini, N. Ulupi, A. Gunawan, C. Sumantri

Abstract

Defensins play roles in innate immunity by exhibiting antimicrobial activity against microbes such as gram-negative and -positive bacteria, viruses, and fungi. This study aimed to identify variants of the Avian β-Defensin 2 (AvBD2) and determine their associations with the concentration of immunoglobulin Y and the titer of Newcastle disease (ND) antibody in IPB-1 chicken. The chicken population used in this study was 21-week-old IPB-D1 chickens (n=90). Variations in AvBD2 were analyzed by direct DNA sequencing. IgY concentration was measured by indirect ELISA, and the titer of ND antibody was measured by the hemagglutination-inhibition test. The AvBD2 association was analyzed by the general linear model procedure and Duncan’s multiple range test. The results revealed 10 SNPs located in intron 1 (3 SNPs), exon 2 (3 SNPs), and intron 2 (4 SNPs). Six of these SNPs were associated with IgY concentration. The CC genotype of g.5002 C>T was associated with IgY concentration and produced the highest mean IgY concentration. This g.5002 C>T mutation results in alanine-to-valine substitutions. The CC genotype of g.5002 C>T could be considered as a criterion for selecting chickens with high IgY concentrations.

References

Al Habib, M. F., S. Murtini, A. Gunawan, N. Ulupi, & C. Sumantri. 2020. Polymorphism of CD1B gene and its association with yolk immunoglobulin (IgY) concentration and Newcastle disease antibody titer in IPB-D1 chicken. Trop. Anim. Sci. J. 43:197-204. https://doi.org/10.5398/tasj.2020.43.3.197
Al Habib, M. F., S. Murtini, L. Cyrilla, I. I. Arief, R. Mutia, & C. Sumantri. 2020. Performa pertumbuhan ayam IPB-D1 pada perlakuan pakan dan manajemen pemeliharaan yang berbeda. J. Agripet. 20:177-186. https://doi.org/10.17969/agripet.v20i2.16375
Allendorf, F. W., & G. H. Luikart. 2007. Conservation and the Genetics of Population. Blackwell Publishing, Oxford.
Cruzat, V. F., M. Krause, & P. Newsholme. 2014. Amino acid supplementation and impact on immune function in the context of exercise. J. Int. Soc. Sports Nutr. 11:61. https://doi.org/10.1186/s12970-014-0061-8
Cuperus, T., M. Coorens, A. van Dijk, & H. P. Haagsman. 2013. Avian host defense peptides. Dev. Comp. Immunol. 41:352-369. https://doi.org/10.1016/j.dci.2013.04.019
Derache, C., V. Labas, V. Aucagne, H. Meudal, C. Landon, A. F. Delmas, T. Magallon, & A. C. Lalmanach. 2009. Primary Structure and Antibacterial Activity of Chicken Bone Marrow-Derived β-Defensins. Antimicrob. Agents Chemother. 53:4647-4655. https://doi.org/10.1128/AAC.00301-09
Guo, C., I. C. McDowell, M. Nodzenski, D. M. Scholtens, A. S. Allen, W. L. Lowe, & T. E. Reddy. 2017. Transversions have larger regulatory effects than transitions. BMC Genomic. 18:394. https://doi.org/10.1186/s12864-017-3785-4
Harter, H. L. 1960. Critical values for Duncan’s new multiple range test. Biometrics 16:671-685. https://doi.org/10.2307/2527770
Hasenstein, J. R., & S. J. Lamont. 2007. Chicken gallinacin gene cluster associated with Salmonella response in advanced intercross line. Avian Dis. 51:561-567. https://doi.org/10.1637/0005-2086(2007)51[561:CGGCAW]2.0.CO;2
Hong, Y. H., W. Song, S. H. Lee, & H. S. Lillehoj. 2012. Differential gene expression profiles of β-defensins in the crop, intestine, and spleen using a necrotic enteritis model in 2 commercial broiler chicken lines. Poult. Sci. 91:1081-1088. https://doi.org/10.3382/ps.2011-01948
Hong, Y., J. Lee, T. H. Vu, S. Lee, H. S. Lillehoj, & Y. H. Hong. 2020. Chicken avian β-defensin 8 modulates immune response via the mitogen-activated protein kinase signaling pathways in a chicken macrophage cell line. Poult. Sci. 99:4174-4182. https://doi.org/10.1016/j.psj.2020.05.027
Hossain, K. M. M., M. Y. Ali, & I. Yamato. 2010. Antibody levels against Newcastle Disease Virus in chickens in Rajshahi and surrounding districts of Bangladesh. Int. J. Biol. 2:1-5. https://doi.org/10.5539/ijb.v2n2p102
Indriani, R. & I. Dharmayanti. 2016. Respon titer antibodi and proteksi virus Newcastle disease genotype I, II, VI dan VII sebagai vaksin terhadap infeksi isolat virus Newcastle disease chicken/Indonesia/GTT/11. Jurnal Biologi Indonesia 12:211-218.
Kapczynski, D. R., C. L. Afonso, & P. J. Miller. 2013. Immune responses of poultry to Newcastle disease virus. Dev. Comp. Immunol. 41:447-453. https://doi.org/10.1016/j.dci.2013.04.012
Kowalczyk, K. J., J. Daiss, J. Halpern, & T. F. Roth. 1985. Quantitation of maternal-fetal IgG transport in the chicken. Immunology. 54:755-762.
Li, P., Y. L. Yin, D. Li, S. W. Kim, & G. Wu. 2007. Amino acids and immune function. Br. J. Nutr. 98:237-252. https://doi.org/10.1017/S000711450769936X
Liu, C., L. Jiang, L. Liu, L. Sun, W. Zhao, Y. Chen, T. Qi, Z. Han, Y. Shao, S. Liu, & D. Ma. 2018. Induction of Avian β-Defensin 2 is possibly mediated by the p38 MAPK signal pathway in chicken embryo fibroblasts after Newcastle disease virus infection. Front. Microbiol. 9:751. https://doi.org/10.3389/fmicb.2018.00751
Lynn, D. J., R. Higgs, S. Gaines, J. Tierney, T. James, A. T. Lloyd, M. A. Fares, G. Mulcahy, & C. O’Farrelly. 2004. Bioinformatic discovery and initial characterisation of nine novel antimicrobial peptide genes in the chicken. Immunogenetics 56:170-177. https://doi.org/10.1007/s00251-004-0675-0
Lynn, D. J., R. Higgs, A. T. Lloyd, C. O’Farrelly, V. Hervé-Grépinet, Y. Nys, F. S. Brinkman, P. L. Yu, A. Soulier, P. Kaiser, G. Zhang, & R. I. Lehrer. 2007. Avian beta-defensin nomenclature: a community proposed update. Immunol. Lett. 110:86-89. https://doi.org/10.1016/j.imlet.2007.03.007
Morammazi, S. & H. Habibi. 2017. Sequence variation in GAL1 and GAL2 genes in Khuzestan local chickens. Eur. Online J. Nat. Soc. Sci. 6:508-515.
Masrurah, I. Khaerunnisa, S. Murtini, & C. Sumantri. 2021. Avian Beta Defensin 2 (AvBD2) gene polymorphism identification in IPB-D1 chicken. JITV 26:82-88. https://doi.org/10.14334/jitv.v26i2.2715
Mukhopadhyay, T. & S. Bhattacharjee. 2016. Genetic diversity: Importance and Measurements. In: A. H. Mir, N. A. Bhat (Eds). Conserving Biological Diversity: A Multiscaled Approach. Research India Publications, New Delhi. p. 251-295.
Munhoz, L. S., G. D. Vargas, G. Fischer, Md Lima, P. A. Esteves, & S. de Oliviera Hübner. 2014. Avian IgY antibodies: Characteristics and applications in immunodiagnostic. Cienc. Rural. 44:153-160. https://doi.org/10.1590/S0103-84782014000100025
Rahman, M., S. Mostafijur, R. Deb, & M. Nooruzzaman. 2017. Evaluation of serum antibody titer level against Newcastle disease virus in vaccinated broiler chickens. Avas 4:94-98.
Rengaraj, D., A. D. Truong, H. S. Lillehoj, J. Y. Han, & Y. H. Hong. 2018. Expression and regulation of avian beta-defensin 8 protein in immune tissues and cell lines of chickens. Asian-Australas. J. Anim. Sci. 31:1516-1524. https://doi.org/10.5713/ajas.17.0836
Sugiarto, H. & P. L. Yu. 2004. Avian antimicrobial peptides: the defense role of beta-defensins. Biochem. Biophys. Res. Commun. 323:721-727. https://doi.org/10.1016/j.bbrc.2004.08.162
Sumantri, C., I. Khaerunnisa, & A. Gunawan. 2020. The genetic quality improvement of native and local chicken to increase production and meat quality in order to build the Indonesian chicken industry. IOP Conf. S. Earth Environ. Sci. 492:012099. https://doi.org/10.1088/1755-1315/492/1/012099
Terada, T., T. Nii, N. Isobe, & Y. Yoshimura. 2018. Changes in the expression of Avian β-defensins (AvBDs) and proinflammatory cytokines and localization of AvBD2 in the intestine of broiler embryos and chicks during growth. J. Poult. Sci. 55:280-287. https://doi.org/10.2141/jpsa.0180022
Ulupi, N., C. Sumantri, & S. Darwati. 2016. Resistance Against Salmonella Pullorum in IPB-D1 Crossbreed, Kampong and Commercial Broiler Chicken. The 1st Conference Technology on Bioscience and Social Science. Universitas Andalas, Padang.
van Dijk, A., E. J. A. Veldhuizen, & H. P. Haagsman. 2008. Avian defensins. Vet. Immunol. Immunopathol. 124:1-18. https://doi.org/10.1016/j.vetimm.2007.12.006

Authors

D. Lestari
Masruroh
I. Khaerunnisa
S. Murtini
N. Ulupi
A. Gunawan
C. Sumantri
ceces@apps.ipb.ac.id (Primary Contact)
LestariD., Masruroh, KhaerunnisaI., MurtiniS., UlupiN., GunawanA., & SumantriC. (2022). Variation and Association of Avian β-Defensin 2 Gene with the Concentration of Immunoglobulin Y and the Titer of Newcastle-Disease Antibody in IPB-D1 Chicken. Tropical Animal Science Journal, 45(2), 121-127. https://doi.org/10.5398/tasj.2022.45.2.121

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