Comparison between the Brix Refractometer and ELISA for Assessment of Colostrum IgG of Montbeliard x Holstein Cross Dairy Cows

N. H. Fahim, T. A. Imbabi


The objectives of the present study were to examine the association between the brix refractometer% and ELISA assays in the assessment of colostrum-IgG content and in studying the factors affecting this content. Contents of IgG in colostrum samples of 132 healthy primiparous and multiparous Montbeliard x Holstein cross dairy cows kept in Egypt were evaluated using ELISA assay and brix refractometer%. The ELISA results showed that the IgG contents in the colostrum samples ranged from 5.96 to 114.84 mg/mL, with a mean of 57.07 mg/mL. Brix% results ranged from 15% to 35%, with a mean of 23.97%. The correlation and regression coefficients between ELISA(IgG) and brix% were significantly high (r=0.68 and r2 =0.59, respectively). Prediction regression equation was ELISA(IgG) = 31.81+1.12(brix refractometer%). The effects of parity and season of calving on both ELISA(IgG) and brix% were significant. ELISA(IgG) and brix% were higher in cows ≥ 3 lactations than those in the first and second lactations. Moreover, summer calving recorded higher ELISA(IgG) and brix% than autumn calving. Either ELISA(IgG) or brix% were not significantly affected by calf’s sex, single/twin births, or history of cows’ diseases. ELISA(IgG) and brix% had a significant positive correlation with calf’s birth weights and gestation lengths, while they recorded a non-significant correlation with dry-period length. It can be concluded that a refractometer is a simple on-farm tool to assess colostrum quality. Its high correlation with the lab analytical assay of ELISA confirms the validity of using refractometers by researchers in colostrum studies.


Angulo, J., L. Miguel Gómez, L. Mahecha, E. Mejía, J. Henao, & C. Mesa. 2015. Calf’s sex, parity and the hour of harvest after calving affect colostrum quality of dairy cows grazing under high tropical conditions. Trop. Anim. Health. Prod. 47:699-705.
Argüello, A., N. Castro, S. Álvarez, & J. Capote. 2006. Effects of the number of lactations and litter size on chemical composition and physical characteristics of goat colostrum. Small Rumin. Res. 64:53-59.
Bartier, A. L., M. C. Windeyer, & L. Doepel. 2015. on-farm tools for colostrum quality measurement. J. Dairy Sci. 98:1878-1884.
Baumrucker, C. R., A. M., Burkett, A. L. Magliaro-Macrina, & C. D. Dechow. 2010. Colostrogenesis: Mass transfer of immunoglobulin G1 into colostrum. J. Dairy Sci. 93:3031-3038.
Baumrucker, C. R., C. D. Dechow, A. L. Macrina, J. J.Gross, & R. M. Bruckmaier. 2016. Mammary immunoglobulin transfer rates following prepartum milking. J. Dairy Sci. 99:9254-9262.
Berry, D. P., F. Buckley, P. Dillon, R. D. Evans, & R. F. Veerkamp. 2004. Genetic relationships among linear type traits, milk yield, body weight, fertility and somatic cell count in primiparous dairy cows. Irish J. Agric. Food Res. 43:161-176.
Bielmann, V., J. Gillan, N. R. Perkins, A. L. Skidmore, S. Godden, & K. E. Leslie. 2010. An evaluation of Brix refractometry instruments for measurement of colostrum quality in dairy cattle. J. Dairy Sci. 93:3713-3721.
Buczinski, S. & J. M. Vandeweerd. 2016. Diagnostic accuracy of refractometry for assessing bovine colostrum quality: A systematic review and meta-analysis. J. Dairy Sci. 99:7381-7394.
Cabral, R. G., C. E.Chapman, K. M. Aragona, E. Clark, M. Lunak, & P. S. Erickson. 2016. Predicting colostrum quality from performance in the previous lactation and environmental changes. J. Dairy Sci. 99:4048-4055.
Conneely, M. D., P. Berry, R. Sayers, J. P. Murphy, I. Lorenz, M. L. Doherty, & E. Kennedy. 2013. Factors associated with the concentration of immunoglobulin G in the colostrum of dairy cows. Animal 7:1824-1832.
Csapo, J., Z. Csapo, T. G. Martin, J. Szentpeteri, & G. Wolf. 1994. Composition of colostrum from goats, ewes and cows producing twins. Int. Dairy J. 4:445-458.
Denholm, K. S., S. McDougall, G. Chambers, & W. Clough. 2018. Factors associated with colostrum quality in individual cows from dairy herds in the Waikato region of New Zealand. N. Z. Vet. J. 66:115-120.
Djoharjani, T., A. Ridhowi, & S. Kemal. 2019. The Effect of Parity on Colostrum Quality of Friesian Holstein Crossbred Cows in Indonesia. IOP Conference Series: Earth and Environmental Science, Vol. 478, The 4th Animal Production International Seminar 24-27 October, Malang, Indonesia.
Dunn, A., A. Ashfield, B. Earley, M. Welsh, A. Gordon, & S. J. Morrison. 2017. Evaluation of factors associated with immunoglobulin G, fat, protein, and lactose concentrations in bovine colostrum and colostrum management practices in grassland-based dairy systems in Northern Ireland. J. Dairy Sci. 100:2068-2079.
Gavin, K., A. Hoffman, J.N. Kiser, M.A.Cornmesser, S. AmirpourHaredasht, B. Martínez-López, J.R. Wenz, & D.A. Moore. 2018. Low colostrum yield in Jersey cattle and potential risk factors. J. Dairy Sci. 101:6388-6398.
Geiger, A. J. 2020. Colostrum: Back to basics with immunoglobulins. J. Anim. Sci. 98:126-132.
Genc, M. 2015. Effect Of Some Environmental Factors on Colostrum Quality and Passive Immunity in Brown Swiss and Holstein Cattle. Ph.D. Thesis, Atatürk Uni., Health Sciences Institute, Erzurum.
Gross, J. J., E.C. Kessler, V. Bjerre-Harpoth, C. Dechow, C. R. Baumrucker, & R. M. Bruckmaier. 2014. Peripartal progesterone and prolactin have little effect on the rapid transport of immunoglobulin G into colostrum of dairy cows. J. Dairy Sci. 97:2923-2931.
Gulliksen, S.M., K.I. Lie, L. Sølverød, & O. Østera. 2008. Risk factors associated with colostrum quality in Norwegian dairy cows. J. Dairy Sci. 91:704-712.
Hoyraz, M., R. Sezer, M. Demirtaş, & A. Koç. 2015. A Research on colostrum quality and constituents of Holstein-Friesian cows. J. Tralleis Elektron. 4:1-7.
Johnsen, J. F., H. Viljugrein, K. E. Boe, S. M. Gulliksen, A. Beaver, A. M. Grøndahl, T. Sivrtsen, & C. M. Mejdell. 2019. A cross-sectional study of suckling calves’ passive immunity and associations with management routines to ensure colostrum intake on organic dairy farms. Acta Vet. Scand. 61:1-10.
Kessler, C., G. C. Pistol, R. M. Bruckmaier, & J. J. Gross. 2020a. Pattern of milk yield and immunoglobulin concentration and factors associated with colostrum quality at the quarter level in dairy cows after parturition. J. Dairy Sci. 103: 965-971.
Kessler, E. C., R. M. Bruckmaier, & J. J. Gross. 2020b. Colostrum composition and immunoglobulin G content in dairy and dual-purpose cattle breeds. J. Anim. Sci. 98:skaa 237.
Le Cozler, Y., R. Guatteo, E. LeDréan, H. Turban, F. Leboeuf, K. Pecceu, & J. Guinard-Flament. 2016. IgG1 variations in the colostrum of Holstein dairy cows. Animal 10: 230-237.
Løkke, M. M., R. Engelbrecht, & L. Wiking. 2016. Covariance structures of fat and protein influence the estimation of IgG in bovine colostrum. J. Dairy Res. 83:58-66.
Maunsell, F. P., D. E. Morin, P. D. Constable, W. L. Hurley, I. McCoy, G. C. Kakoma, & R. E. Isaacson. 1998. Effects of mastitis on the volume and composition of colostrum produced by Holstein cows. J. Dairy Sci. 81:1291-1299.
Mayasari, N., G. de Vries Reilingh, M. G. Nieuwland, G. J. Remmelink, H. K. Parmentier, B. Kemp, & A. T. van Knegsel. 2015. Effect of maternal dry period length on colostrum immunoglobulin content and on natural and specific antibody titers in calves. J. Dairy Sci. 98:3969-3979.
Morrill, K. M., E. Conrad, A. Lago, J. Campbell, J. Quigley, & H. Tyler. 2012. Nationwide evaluation of quality and composition of colostrum on dairy farms in the United States. J. Dairy Sci. 95:3997-4005.
Morrill, K. M., K. E. Robertson, M. M. Spring, A. L. Robinson, & H. D. Tyler. 2015. Validating a refractometer to evaluate immunoglobulin G concentration in Jersey colostrum and the effect of multiple freeze-thaw cycles on evaluating colostrum quality. J. Dairy Sci. 98:595-601.
Morrow, D. A. 1976. Fat cow syndrome. J. Dairy Sci. 59:1625-1629.
Puppel, K., M. Gołębiewski, G. Grodkowski, P. Solarczyk, P. Kostusiak, M. Klopčič, & T. Sakowski. 2020. Use of somatic cell count as an indicator of colostrum quality. PLoS ONE 15:1-15.
Quigley, J. D., A. Lago, C. Chapman, P. Erickson, & J. Polo. 2013. Evaluation of the Brix refractometer to estimate immunoglobulin G concentration in bovine colostrum.J. Dairy Sci. 96:1148-1155.
Shivley, C. B., J. Lombard, N. Urie, D. Haines, R. Sargent, C. Kopral, T. Earleywine, J. Olson, & F. Garry. 2018. Pre weaned heifer management on US dairy operations: Part II. Factors associated with colostrum quality and passive transfer status of dairy heifer calves. J. Dairy Sci. 101:9185-9198.
Silva-Del-Río, N., D. Rolle, A. García-Muñoz, S. Rodríguez Jiménez, A. Valldecabres, A. Lago, & P. Pandey. 2017. Colostrum immunoglobulin G concentration of multiparous Jersey cows at first and second milking is associated with parity, colostrum yield, and time of first milking, and can be estimated with Brix refractometry. J. Dairy Sci. 100:5774-5781.
Shoshoni, E., S. Rozen, & J. J. Doekes. 2014. Effect of a short dry period on milk yield and content, colostrum quality, fertility, and metabolic status of Holstein cows. J. Dairy Sci. 97:2909-2922.
Sutter, F., S. Borchardt, G. M. Schuenemann, E. Rauch, M. Erhard, & W. Heuwieser. 2019. Procedures after calving to improve harvesting of high-quantity and high-quality colostrum. J. Dairy Sci. 102: 9370-9381.
Turini, L., G. Conte, F. Bonelli, M. Sgorbini, A. Madrigali, & M. Mele. 2020. The relationship between colostrum quality, passive transfer of immunity and birth and weaning weight in neonatal calves. Livest. Sci. 238:1-4.
Verweij, J. J., A. P. Koets, & S. W. F. Eisenberg. 2014. Effect of continuous milking on immunoglobulin concentrations in bovine colostrum. Vet. Immunol. Immunopathol. 160:225-229.
Zentrich, E., M. Iwersen, M. C. Wiedrich, M. Drillich, & D. Klein-Jöbstl. 2019. Short communication: Effect of barn climate and management-related factors on bovine colostrum quality. J. Dairy Sci. 102:7453-7458.


N. H. Fahim (Primary Contact)
T. A. Imbabi
FahimN. H., & ImbabiT. A. (2021). Comparison between the Brix Refractometer and ELISA for Assessment of Colostrum IgG of Montbeliard x Holstein Cross Dairy Cows. Tropical Animal Science Journal, 44(3), 356-362.

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