Reproductive Responses, Metabolic Disorder, Ruminal Fermentation Characteristics, and Milk Production of Postpartum Holstein Cows Fed Steam Flaked of Corn and Barley Grains

N. Ahmadijoo, H. Mansoori-Yarahmadi, J. Fakhraei, M. Changizi


This study evaluated the effects of steam flaked of corn and barley grains on the reproductive response, ruminal fermentation characteristics, metabolic disorder, and milk production of postpartum Holstein cows. A total of 40 postpartum Holstein dairy cows were divided into 4 groups (n=10) and fed 1) ground grains (control), 2) steam flacked corn+milled barley, 3) steam flacked barely+milled corn, and 4) steam flaked corn and barley. The animals were treated for 0-30, 30-60, 60-90, and 90-120 days post-calving. Milk yield and compositions, total conception rate, endometritis, pyometra, ruminal fermentation characteristics, ketosis, and acidosis were assessed. This study was conducted in a completely randomized design. The results showed that dietary inclusion of steam flaked grains improved milk yield and compositions during the experimental period compared to the control group (p<0.05), except for cows treated for 0-30d days post-calving. Cows fed steam flaked grains showed a lower occurrence of reproduction and metabolic disorders compared to the control group (p<0.01). Cows fed a combination of steam flaked grains showed better pregnancy rates in the first 100 days and open days of postpartum cows compared to cows in control and a single form of steam flaked barley or corn (p<0.05). Total VFA, acetate, propionate, butyrate, and valerate were higher in cows fed steam flaked compared to the control group (p<0.05). Feeding steam flaked grains is advised for improving reproductive performance, milk variables, and rumen fermentation profiles in dairy cows.


Ansari-Lari, M., M. Kafi, M. Sokhtanlo, & H. N. Ahmadi. 2010. Reproductive performance of Holstein dairy cows in Iran. Trop. Anim. Health. Prod. 42: 1277-1283.

Atmakusuma, J., B.M. Sinaga, N. Kusnadi, & I. K. Kariyasa. 2019. The impact of external and internal factors on the dairy farmer’s household economics. Trop. Anim. Sci. J. 42: 245-252.

Bramley, E., I. J. Lean, W. J. Fulkerson, M. A. Stevenson, A. R. Rabiee, & N. D. Costa. 2008. The definition of acidosis in dairy herds predominantly fed on pasture and concentrates. J. Dairy. Sci. 91: 308-321.

Cooke, K. M., J. K. Bernard, & J. W. West. 2008. Performance of dairy cows fed annual ryegrass silage and corn silage with steam-flaked or ground corn. J. Dairy. Sci. 91: 2417-2422.

Corato, A., S. Segato, & I. Andrighetto. 2005. Effects of extruded corn on milk yield and composition and blood parameters in lactating dairy cows. Ital. J. Anim. Sci. 4: 166-168.

Dehghan-banadaky, M., R. Corbett, & M. Oba. 2007. Effects of barley grain processing on productivity of cattle. Anim. Feed. Sci. Technol. 137: 1-24.

Dehkordi, A. J., & Z. K. Dehkordi. 2011. Occurrence of metabolic alkalosis in rumen lactic acidosis: a review. Compar. Clin. Pathol. 20: 1-3.

Dobson, H., S. L. Walker, M. J. Morris, J. E. Routly, & R. F. Smith. 2008. Why is it getting more difficult to successfully artificially inseminate dairy cows?. Anim. 2: 1104-1111.

Duru, S. & S. Can Baycan. 2019. Change of daily milk yield during estrous period in Holstein cattle raised under Mediterranean climate. Trop. Anim. Health. Produc. 51: 1571-1577.

Ferguson, J. D., G. Azzaro, & G. Licitra. 2006. Body condition assessment using digital images. J. Dairy. Sci. 89: 3833-3841.

Ghorbani, G. R., F. Ahmadi, & M. Haidary. 2016. Effect of steam-flaked and ground corn with different particle size on dairy cow performance with high-concentrate diet. J. Anim. Sci. 94: 701.

Garnsworthy, P. C., J. G. Gong, D. G. Armstrong, J. R. Newbold, M. Marsden, S. E. Richards, G. E. Mann, K. D. Sinclair, & R. Webb. 2008. Nutrition, metabolism, and fertility in dairy cows: 3. Amino acids and ovarian function. J. Dairy. Sci. 91: 4190-4197.

Güntner, A. T., J. F. Kompalla, H. Landis, S. J. Theodore, B. Geidl, N. A. Sievi, M. Kohler, S. E. Pratsinis, & P. A. Gerber. 2018. Guiding ketogenic diet with breath acetone sensors. Sensors. 18: 3655.

Koening, K. K. Beauchemin, & W.Z. Yang. 2013. Processing feed grain: factors affecting the effectiveness of grain processing for beef and dairy cattle production. Processing of 34th western nutrition conference-processing, performance and profit, Saskatoon, Saskatchewan, Canada. p. 62-73.

Koing, K., & K. Beauchemin. 2011. Optimum extent of barley grain processing and barley silage proportion in feedlot cattle diets: Growth, feed efficiency and fecal characteristics. Canad. J. Anim. Sci. 91: 411-422.

Kokić, B., J. Lević, M. Chrenková, Z. Formelová, M. Poláčiková, M. Rajský, & R. Jovanović. 2013. Influence of thermal treatments on starch gelatinization and in vitro organic matter digestibility of corn. Food. Feed. Res. 40 (Suppl. 2): 93-99.

Lucy, M. C. 2001. Reproductive loss in high-producing dairy cattle: where will it end?. J. Dairy. Sci. 84: 1277-1293.

Moharrery, A., G. Himoller, & M.R. Weisbjerg. 2017. The effect of rolled barley, sodium hydroxide-treated wheat or maize cob silage on digestive enzymes activity in the alimentary tract of dairy cows. J. Anim. Feed. Sci. 26: 303-310.

Nikkhah. A., M. Alikhani, & H. Amanlou. 2004. Effects of feeding ground or steam-flaked broom sorghum and ground barley on performance of dairy cows in midlactation. J. Dairy. Sci. 87: 122-130.

Nordlund, K. & E. Garrett. 1994. Rumenocentesis: a technique for collecting rumen fluid for the diagnosis of subacute rumen acidosis in dairy herds. Bovine. Practitioner. 28: 109-112.

NRC. 2001. Nutrient Requirements of Dairy Cattle. 7th rev. edn. Natl Acad Press, Washington, DC.

Opsomer, G. & A. Kriuf. 2009. Metritis and endometritis in high yielding dairy cows. Vlaams. Diergeneeskundig. Tijdschrift. 78: 83-88.

Razavi, S. A., M. Pourjafar, A. Hajimohammadi, R. Valizadeh, A. A. Naserian, R. Laven, & K. R. Mueller. 2019. Effects of dietary supplementation of bentonite and Saccharomyces cerevisiae cell wall on acute-phase protein and liver function in high-producing dairy cows during transition period. Trop. Anim. Health. Prod. 51: 1225-1237.

Razzaghi, A., M. Larsen, P. Lund, & M. R. Weisbjerg. 2016. Effect of conventional and extrusion pelleting on in situ ruminal degradability of starch, protein and fiber in cattle. Livest. Sci. 185: 97-105.

Safaei, K. H., G. R. Ghorbani, M. Alikhani, A. Sadeghi Sefidmazgi, W.Z. Yang, & F. Mohammadi. 2016. Effects of processing method, steaming duration and roller setting distance on ruminal degradability of barley grain. J. Rum. Res. 3: 103-126.

Safaei, K. H., G. R. Ghorbani, M. Alikhani, A. Sadeghi Sefidmazgi, & W. Z. Yang. 2017. Response of lactating dairy cows to degree of steam-flaked barley grain in low-forage diets. J. Anim. Physiol. Anim. Nutr. 101: 87-97.

Safaei, K.H. & W.Z. Yang. 2017. Effects of Grain Processing with Focus on Grinding and Steam-Flaking on Dairy Cow Performance. In: Herbivores. pp. 118-131.

Santos, F. A. P., J. T. Huber, G. B. Theurer, R. S. Swingle, Z. Wu, J. M. Simas, K. H. Chen, S. C. Chan, J. Santos, & E. J. DePeters. 1997. Comparison of barley and sorghum grain processed at different densities for lactating cows. J. Dairy. Sci. 80: 2098-2103.

Sheldon, I. M., E. J. Williams, A. N. Miller, D. M. Nash, & S. Herath. 2008. Uterine diseases in cattle after parturition. Vet. J. 176: 115-121.

Sirotkin, A.V., A.V. Makarevich, P. Makovicky, & E. Kubovicova. 2013. Ovarian, metabolic and endocrine indexes in dairy cows with different body condition scores. J. Anim. Feed. Sci. 22: 316-322.

Tajik, J., M. G. Nadalian, A. Raoofi, G. R. Mohammadi, & A. R. Bahonar. 2009. Prevalence of subacute ruminal acidosis in some dairy herds of Khorasan Razavi province northeast of Iran. Iran. J. Vet. Res. 1: 28-32.

Theurer, C., H. Huber, & F. Santos. 1995. Feeding and managing for maximal milk protein, Southwest Nutrition and Management Conference.

Van Vuuren, A. M., V. A. Hindle, A. Klop, & J. W. Cone. 2010. Effect of maize starch concentration in the diet on starch and cell wall digestion in the dairy cow. J. Anim. Physiol. Anim. Nutr. 94: 319-329.

Williams, E. J., D. P. Fischer, D. U. Pfeiffer, G. C. England, D. E. Noakes, H. Dobson, & I. M. Sheldon. 2005. Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle. Theriogenology 63: 102-117.

Xu, N., J. Liu, & P. Yu. 2018. Alteration of biomacromolecule in corn by steam flaking in relation to biodegradation kinetics in ruminant, revealed with vibrational molecular spectroscopy. Spectrochimica. Acta Part A. Mol. Bimolecul. Spectroscopy. 19: 491-497.

Yang, W.Z., M. Oba, & T. A. Mc Allister. 2013. Quality and precision processing of barley grain affected intake and digestibility of dry matter in feedlot steers. Canad. J. Anim. Sci. 93: 251-260.

Zinn, R., F. Owens, & R. Ware. 2002. Flaking corn: processing mechanics, quality standards, and impacts on energy availability and performance of feedlot cattle. J. Anim. Sci. 80 (Suppl. 5): 1145-1156.


N. Ahmadijoo
H. Mansoori-Yarahmadi (Primary Contact)
J. Fakhraei
M. Changizi
AhmadijooN., Mansoori-YarahmadiH., FakhraeiJ., & ChangiziM. (2020). Reproductive Responses, Metabolic Disorder, Ruminal Fermentation Characteristics, and Milk Production of Postpartum Holstein Cows Fed Steam Flaked of Corn and Barley Grains. Tropical Animal Science Journal, 43(3), 240-247.

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