Polymorphism of L-FABP (SNP g. 1593 C>T) Gene and Its Association with Fatty Acid Composition, Carcass, and Meat Quality in Cihateup Duck

  • A. S. Mentari Graduate School of IPB University (Bogor Agricultural University)
  • A. Gunawan Department of Animal Production and Technology, Faculty of Animal Science, IPB University
  • R. R. Noor Department of Animal Production and Technology, Faculty of Animal Science, IPB University
Keywords: L-FABP gene, fatty acid, meat quality, Cihateup duck

Abstract

Fatty acid composition, carcass, and meat quality are quantitative traits which are controlled by several genes or polygenic. One of the genes that plays important role in the fatty acid in meat quality and fatty acid composition is liver-type fatty acid-binding protein (L-FABP). The aims of this study were to identify single nucleotide polymorphism (SNP) of liver-fatty acid binding protein (L-FABP) gene and its association with fatty acid, carcass, and meat quality traits in cihateup duck. Cihateup ducks were originated from Tasikmalaya, West Java, Indonesia. The study used a total of 98 ducks aged of 12 weeks old with the average body size of 1.4±0.12 kg for PCR-RFLP analyses and 76 ducks for the association analyses. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) was used to identify SNP g. 1593 (C>T) of L-FABP gene. The associations of L-FABP genotypes with fatty acid, carcass, and meat quality traits were performed using T-test procedures. The result showed that the SNP of L-FABP gene was polymorphic with three genotypes (CC, CT, and TT). The Chi-square test revealed that the locus of L-FABP (g. 1593 C>T) was in Hardy - Weinberg equilibrium. L-FABP gene was significantly associated (p<0.01) with the carcass portions, providing neck and percentage of neck as well as significantly associated (p<0.05) with saturated fatty acids, i.e., lauric acid (C 12:0) and palmitic acid (C 16:0); polyunsaturated fatty acid, i.e., eicosadienoic acid (C 20:2); carcass portions, i.e. neck and percentage of neck. The SNP g. 1593 (C>T) of L-FABP gene may be a useful marker for selecting and producing duck meat having desirable fatty acids and carcass and meat quality.

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References

AMSA. 2012. Meat Color Measurement Guidlines. American Meat Science Association, USA.

Anggraeni, A. Gunawan, Rukmiasih, T. Suryati, & C. Sumantri. 2017. Association and expression analyses of the duck FMO3 gene in relation to fatty acid composition. Int. J. Poult. Sci. 16: 486-493. https://doi.org/10.3923/ijps.2017.486.493

Atshaves, B. P., G. G. Martin, H. A. Hostetler, A. L. McIntosh, A. B. Kier, & F. Schroeder. 2010. Liver fatty acid binding protein and obesity. J. Nutr. Biochem. 11: 1015-1032. https://doi.org/10.1016/j.jnutbio.2010.01.005

Bohle, H. M. & T. Gabaldon. 2012. Selection of marker genes using whole-genome DNA polymorphism analysis. Evo. Bioinfo. 8: 161-169. https://doi.org/10.4137/EBO.S8989

Briggs, M.A., K. S. Petersen, & P. M. Kris-Etherton. 2017. Saturated fatty acids and cardiovascular disease: replacements for saturated fat to reduce cardiovascular risk. Healthcare. 5: 1-29. https://doi.org/10.3390/healthcare5020029

Costa, J. B., R. L. Oliveira, T. M. Silva, A. M. Barbosa, M. S. Borja, C. B. de Pellergrini, V. da S. Oliveira, R. D. X. Ribeiro, & L. R. Bazerra. 2018. Fatty acid, physicochemical composition and sensory attributes of meat from lambs fed diets containing licuri cake. PloS One. 11: 1-15. https://doi.org/10.1371/journal.pone.0206863

Direktorat Jendral Peternakan dan Kesehatan Hewan Kementerian Pertanian. 2017. Statistik dan Kesehatan Hewan. Kementrian Pertanian RI, Jakarta.

Ebrahami, M., M. A. Rajion, S. Jafari, M. F. Jahromi, E. Oskoueian, A. Q. Sazili, Y. M. Goh, & M. H. Ghaffari. 2018. Effect of dietary n-6: n-3 polyunsaturated fatty acid ration on meat quality, carcass characteristics, tissue fatty acid profiles, and expression of lipogenic genes in growing goats. PloS One. 8: 1-21. https://doi.org/10.1371/journal.pone.0188369

Ferre, M. G., G. Zong, W. C. Willet, P. L. Zock, A. J. Wanders, F. B. Hu, & Q. Sun. 2019. Associations of monounsaturated fatty acids from plant and animal sources with total and cause-specific mortality in two US prospctive cohrot studies. Circ. Res. 124: 1266 - 1275. https://doi.org/10.1161/CIRCRESAHA.118.313996

Gunawan, A., C. Sumantri, & R. Juniarti. 2017. Gen dan Keragaman Genetik Ternak. IPB Pr, Bogor.

Hartl, D.L. & A.G. Clark. 1997. Principle of Population Genetic. Sinauer Associates, Sunderland.

He, J., J. Chen, L. Lu, Y. Tian, Z. Tao, D. Wang, J. Li, G. Li, J. Shen, Y. Fu, & D. Niu. 2011. A novel snp of liver type fatty acid-binding protein gene in duck and its associations with the intramuscular fat. Mol. Biol. Rep. 39: 1073-1077. https://doi.org/10.1007/s11033-011-0833-z

He, J., Y. Tian, J. Li, J. Shen, Z. Tao, Y. Fu, D. Niu, & L. Lu. 2012. Expression pattern of L-FABP gene in different tissues and its regulation of fat metabolism-related genes in duck. Mol. Biol. Rep. 40: 189-195. https://doi.org/10.1007/s11033-012-2048-3

Jump, D.B., S. Tripathy, & C. M. Depner. 2013. Fatty acid-regulated transcription factors in the liver. Annu. Rev. Nutr. 33: 249-269. https://doi.org/10.1146/annurev-nutr-071812-161139

Kerry, J. P. & D. Ledward. 2009. Improving The Sensory and Nutritional Quality of Fresh Meat. CRC Press (ENG), Cambridge. https://doi.org/10.1533/9781845695439

Martin, G. G., A. L. McIntosh, H. Huang, S. Gupta, B. P. Atshaves, K. K. Landrock, D. Landrock, A. B. Kier, & F. Schroeder. 2013. Human liver fatty acid binding protein (L-FABP) T94A variant altres structure, stability, and interaction with fibrates. Biochem. 51: 9347-9357. https://doi.org/10.1021/bi401014k

Nei, M. & S. Kumar. 2000. Molecular Evolution and Phylogenetics. Oxford Univ Pr., New York.

Noor, R. R. 2010. Genetika Ternak. Penebar Swadaya, Jakarta.

Orellana, C., F. Pena, A. Garcia, J. Perea, J. Martos, V. Domenech, & R. Acero. 2009. Carcass characteristics, fatty acid composition, and meat quality of Criollo Argentino and Braford steers raised on forage in a semi-tropical region of Argentina. Meat Sci. 81: 57-64. https://doi.org/10.1016/j.meatsci.2008.06.015

Poulsen, N. A., F. Gustavsson, M. Glantz, M. Paulsson, L. B. Larsen, & M. K. Larsen. 2012. The influence of feed and herd on fatty acid composition in 3 dairy breeds (danish holstein, danish jersey, and swedish red). J. Dairy. Sci. 95: 6362-6371. https://doi.org/10.3168/jds.2012-5820

Praagman, J., L. E. T. Vissers, A. A. Mulligan, A. S. D. Laursen, J. W. J. Beulens, Y. T. van der Schouw, N. J. Wareham, C. P. Hansen, K. T. Khaw, M. U. Jakobsen, & I. Sluijs. 2019. Consumption of individual saturated fatty acids and the risk of myocardial infarction in a UK and Danish cohort. Int. J. Card. 279: 18-26. https://doi.org/10.1016/j.ijcard.2018.10.064

Roussell, M. A., A. M. Hill, T. L. Gaugler, S. G. West, J. P. V. Heuvel, P. Alaupovic, P. J. Gillies, & P. M. Kris-Etherton. 2012. Beef in optimal lean diet study: effect on lipids, lipoproteins, and apolipoprotein. Am. J. Clin. Nutr. 95: 9-16. https://doi.org/10.3945/ajcn.111.016261

Sambrook, J., J. F. Fritsch, & T. Maniatis. 1989. Moleculer Cloning: A Laboratory Manual. Cold Spring Harbour Lab Pr., New York.

Viljoen, G.J., H.N. Louis, & R.C John. 2005. Molecular Diagnostic PCR Handbook. Springer, Dordrecht, Netherland.

Virtanen, J.K., J. Mursu, T. P. Tuomainen, & S. Voutilainen. 2014. Dietary fatty acids and risk of coronary heart disease in men: the kuopio ischemic heart disease risk factor study. Arterioscler. Thromb. Vasc. Biol. 34:1-9. https://doi.org/10.1161/ATVBAHA.114.304082

Wideman, N., C. A. O’bryan, & P. G. Crandall. 2016. Factors affecting poultry meat colour and consumer preferences - A review. World’s Poult. Sci. J. 72: 353-366. https://doi.org/10.1017/S0043933916000015

Zhang, Y.H., L.S. Dai, T.H. Ma, S.Z. Wang, J. Guo, F.J. Li, S.M. Zhang, B.X. Sun, D.F. Liu, Y. Gao, & J.B. Zhang. 2013. Association of T1740C polymorphism of L-FABP with meat quality traits in Junmu No.1 white swine. Gen. Mol. Researc. 12: 235-241. https://doi.org/10.4238/2013.January.30.9

Published
2020-06-03
How to Cite
Mentari, A. S., Gunawan, A., & Noor, R. R. (2020). Polymorphism of L-FABP (SNP g. 1593 C&gt;T) Gene and Its Association with Fatty Acid Composition, Carcass, and Meat Quality in Cihateup Duck. Tropical Animal Science Journal, 43(2), 103-109. https://doi.org/10.5398/tasj.2020.43.2.103