Jurnal Sumberdaya Hayati

Quantitative Analysis of Phytochemical Compounds and Antihyperglycemic Potential of Robusta Coffee from West Lampung

Fri, 15 Mar 2024 11:02:27 +0700

West Lampung Regency in Lampung Province produces high quality robusta coffee with a distinct, strong bitter flavour. Bitter flavour indicates the amount of bioactive substances produced by plants called phytochemicals. The aim of this study is to analyse the phytochemical compounds and antihyperglycemic potential of robusta coffee beans from West Lampung, Indonesia. Quantitative phytochemical analysis was carried out using the thin layer chromatography method and spectrophotometry. Animal experimental design using robusta coffee on hyperglycemia conditions in mice which was induced by alloxan 170 mg/kgBW subcutaneously and given by 1 ml of brewed robusta coffee for 10 days. The robusta coffee from West Lampung contained total caffeine 4,014.87 µg/g, total flavonoid content (TFC) 93.6 mg quercetin equivalent per gram of sample, with total alkaloid content (TAC) 0.848 mg of quinine equivalent per gram of sample, total tannin content (TTC) 182.3 mg of tannic acid equivalent per gram of sample, and total saponin content (TSC) 24.2 mg of quillaja bark equivalent per gram of sample. The administration of 1 ml/mice/day of robusta coffee brewed for 10 days did not show a decrease in blood sugar level in hyperglycemic mice, due to the short duration of the study, so the role of coffee in decreasing hyperglycemia conditions has not been optimally observed.

The Potential of Mycofoam as a Biocomposite Material with Various Substrate and Mushroom Compositions

Revelo Eved Christos, Anastasia Hartanti, Dionysius Subali — Fri, 15 Mar 2024 11:14:47 +0700

Mycofoam is a biocomposite composed of mycelial filaments that bind to the host substrate, which can replace polystyrene. This research aims to determine the most appropriate formulation of the growing medium and mushroom to be used in mycofoam products based on some parameters. Composition is one of the main factors for selecting the most appropriate mycofoam to replace polystyrene. On the other hand, the quality of mycofoam can be assessed through its strength and water resistance. The mushroom mycelium used in this study came from Pleurotus ostreatus and Lentinula edodes. Besides, the growing medium formulation comprises five different compositions, each containing different proportions of sawdust and bagasse. This research was composed of preparation of growing medium, spawn inoculation, molding, heating, testing, and data analysis. Based on the results, the combination of 100 percent sawdust and L. edodes was the most appropriate choice compared to other formulations based on strength and appearance. The results of the water absorption test showed that all mycofoam formulations were not resistant to water, bio-based coating can be used to overcome this problem.

Potensi Cadangan Biji Tumbuhan Bawah Pasca Erupsi Merapi di Taman Nasional Gunung Merapi

Anisa Fadilah, Sulistijorini, Nunik Sri Ariyanti — Mon, 18 Mar 2024 08:45:18 +0700

The 2010 eruption of Mount Merapi caused the ecosystem of Mount Merapi National Park had damage. The recovery of the surrounding ecosystem was called succession . Soil seed bank can be a seed resource for the succession. This research was conducted to analyze vegetation composition and explore the potential seed bank of understorey plants. The vegetation analysis was done using a quadratic method in the three locations: highly damaged, moderately damaged, and lightly damaged. A 1×1 m² plot was used to collect 9 soil samples from each location. The soil samples were stored in a greenhouse for the germination test. Results showed that Poaceae dominated the vegetation in three locations. The vegetation in moderately and lightly damaged locations has a higher score in diversity and evenness than that in highly damaged locations. The seed bank results showed approximately 34 understorey plants from 14 families dominated by Poaceae. The similarity between the standing aboveground vegetation and soil seed banks in the three locations was low.

Aktivitas Antibakteri dan Antioksidan dari Senyawa Bioaktif Asal Bakteri Endofit Tanaman Nyatoh (Palaquium amboinense B.)

Tue, 19 Mar 2024 08:24:16 +0700

Nyatoh plant (Palaquium amboinense B.) is native to Indonesia which is known to have the ability to produce potential secondary metabolites. The aim of this study was to evaluate the antibacterial and antioxidant activities from bioactive compounds produced by endophytic bacteria from nyatoh leaves. Five endophytic bacteria were tested against 4 targeted bacteria including Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilis ATCC 19659, and Staphylococcus aureus ATCC 6538. Bacterial isolate which had the strongest antibacterial activity was then fermented and extracted. The corresponding extract was further tested on disc diffusion method, determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values, antioxidant evaluation, and analyzed for its bioactive compound components via GC-MS analysis. The results showed that one isolate, namely D1 can produce secondary metabolite which act as antibacterial and antioxidant potentials. This corresponding extract has the strongest MIC and MBC against B. subtilis with value of 39.5 and >78.1 µg/ml and exhibited antioxidant activity against 2,2-difenil-1-pikrilhidrazil (DPPH) with inhibition value of 85.37%. Based on GC-MS analysis, secondary metabolites derived from this potential extract contained some major active constituents including Tributyl acetylcitrate and bis (2-ethylhexyl) ester which might act as antibacterial and antioxidant agents.

Pengaruh Status Gizi terhadap Jangka Reproduksi Perempuan pada Tiga Kecamatan di Kabupaten Lampung Selatan

Winati Nurhayu, Syefia Azmi Restina, Gres Maretta — Tue, 19 Mar 2024 08:42:18 +0700

The maturity of women's reproductive organs can be characterized by the onset of the first menstruation, known as menarche, while the concluding phase is referred to as menopause. The time frame spanning from menarche to menopause is known as the reproductive period. One crucial factor influencing the reproductive period is nutritional status. Optimal body fat reserves tend to extend the reproductive period, whereas inadequate reserves can have the opposite effect. Notably, women residing in urban areas often experience a more extended reproductive period compared to their rural counterparts. The aim of this study was to assess the reproductive period of women in three sub-districts in South Lampung and to analyze the potential impact of nutritional status on the individual reproductive period. The study was conducted between January and April 2023 in the sub-districts of Jati Agung, Natar, and Kalianda. Data was gathered from a total of 57 respondents through interviews and physical measurements. Nevertheless, only 18 respondents met the criteria and were further analyzed. Nutritional status was evaluated using the body mass index (BMI) and percent body fat (PBF). The findings revealed that the average reproductive period for women in the three South Lampung sub-districts was 37.06 years. The average age for menarche was 14.22 years, while the average age for menopause was 51.28 years. However, the study did not find a significant correlation between nutritional status and the reproductive period of women in the three examined sub-districts of South Lampung.