@article{Slamet_Aslianti_2016, title={DEVELOPMENT OF DIGESTIVE ENZYMES ACTIVITY ON BLACK SADDLED CORAL GROUPER (Plectropomus laevis) LARVAE}, volume={8}, url={https://jurnal.ipb.ac.id/index.php/jurnalikt/article/view/12427}, DOI={10.29244/jitkt.v8i1.12427}, abstractNote={<p align="center"><strong><em>ABSTRACT</em></strong></p><p><em>Protease, amylase, and lipase enzymes were used as biological indicators to me</em><em>a</em><em>sure larvae’s food digestion. The aim of this study was to describe digestive enzyme activity development of black saddled coral grouper (<span style="text-decoration: underline;">Plectropomus</span> <span style="text-decoration: underline;">laevis</span>) larvae. The just hatching larvae were reared in concrete tank 2x3x1 m<sup>3</sup> with stocking density of 10 larvae/L. In the larval rearing media were added with <span style="text-decoration: underline;">Nanochloropsis</span> <span style="text-decoration: underline;">oculata</span> at 10<sup>5 </sup>cel/ml start</em><em>ed</em><em> from 1 day old (D-1). The larvae </em><em>was </em><em>fed with rotifer (<span style="text-decoration: underline;">Brachionus</span> <span style="text-decoration: underline;">rotundiformis</span>)</em> <em>at 10-20 ind/ml start</em><em>ed</em><em> from D-2 and artificial diet from D-10. To anal</em><em>y</em><em>ze  the protease, amylase, and lipase enzymes activities, </em><em>samples </em><em>were take</em><em>n</em><em> 0.5-1 g or about 1000 larvae on D-3, D-4, D-6, D-8 and 500 larvae on D-10, D-12, D-16, and D-20. The result showed that the protease, amylase, and lipase enzymes activity were positively correlated with the growth. Digestive enzymes activity was increased when larvae started on endogenous feed</em><em>ing</em><em> (D-3), become down on D-6</em><em>,</em><em> stab</em><em>le</em><em> on D-8 to D-10</em><em>, </em><em>increased on D-12, and </em><em>reached the </em><em>highest </em><em>level </em><em>on D-16. Digestive enzymes activity was higher when larvae started feeding with artificial diet compared to the larvae before feeding with artificial diet.</em></p><p><em> </em><strong><em>Keywords:</em></strong><em>  digestive enzymes, development, larvae, blacksaddled coral grouper</em></p&gt;}, number={1}, journal={Jurnal Ilmu dan Teknologi Kelautan Tropis}, author={Slamet, Bejo and Aslianti, Titiek}, year={2016}, month={Nov.}, pages={1-10} }