Konfirmasi Toleransi Galur-galur Padi terhadap Cekaman Kekeringan secara Molekuler

Rina Hapsari Wening; Willy Bayuardi Suwarno; Bambang Sapta Purwoko; Indrastuti Apri Rumanti; Amy Estiati

doi:10.24831/jai.v49i2.36565

Rina Hapsari Wening Balai Besar Penelitian Tanaman Padi, Jl. Raya 9, Sukamandi, Subang 41256, Jawa Barat, Indonesia
Willy Bayuardi Suwarno Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
Bambang Sapta Purwoko Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
Indrastuti Apri Rumanti Balai Besar Penelitian Tanaman Padi, Badan Litbang Pertanian Jl. Raya IX Sukamandi, Subang 41256, Jawa Barat, Indonesia
Amy Estiati Lembaga Ilmu Pengetahuan Indonesia, Jl. Raya Jakarta-Bogor No. Km 46, Cibimong, Bogor 16911, Indonesia

DOI: https://doi.org/10.24831/jai.v49i2.36565

Abstract

Phenotypic selection, especially for abiotic stresses, is often challenging. Molecular selection activities are needed to identify at the molecular level that a tolerant trait is genotypically proven. This study aimed at confirming the drought tolerance of rice lines using SSR markers RM164, RM228, RM248, dan RM328. The study was conducted at the LIPI Biotechnology Laboratory at Cibinong, Bogor, Indonesia from November to December 2017, using the polymerase chain reaction (PCR) technique. The results showed that the RM228 marker was associated with the character of leaf rolling scores, panicle length, number of tillers, number of filled grains per panicle, and productivity. This marker is therefore potential to be used for drought tolerance selection. Seven of the ten lines that indicated as tolerant at generative phase drought stress based on artificial screening, were confirmed molecularly, which were in the same group with the tolerant check ‘Limboto’. The seven lines were B13983E-KA-12-2, B13507E-MR-19, B14366E-KY-50, BP20452e-PWK-0-SKI-1-1, BP20452e-PWK-0-SKI-2-4, BP20452e-PWK-0-SKI-3-3, and BP29790d-PWK-3-SKI-1-5.

Keywords: drought tolerant, marker, polymerase chain reaction