Pengaruh Suhu Ekstraksi terhadap Aktivitas Antioksidan Ekstrak Metanolik Eucheuma spinosum

Effect of Extraction Temperature on Antioxidant Activity of Methanolic Extract of Eucheuma spinosum

Keywords: DPPH, FRAP, red seaweed, total flavonoids, total phenolic content

Abstract

Eucheuma spinosum is red algae that is widely cultivated and is also one of the superior commodities because it has phenolic and flavonoid compounds that can act as antioxidants. Phenolic content and flavonoids in E. spinosum can be obtained through a maceration process with methanol as a solvent which is generally carried out at room temperature. The extraction process to obtain maximum yield can be accelerated by increasing the extraction temperature. This research aims to determine the antioxidant activity of E. spinosum extracted using methanol at temperatures of 55, 65, and 75 °C. Before extraction, the seaweed was washed and dried in an oven at 40 °C for 24 hours. Extraction was carried out with 80 % methanol in a ratio of 1:10 (w/v). The parameters analyzed in this research were yield, phytochemical test, total phenolic content (TPC), total flavonoids, antioxidant activity including DPPH and FRAP. The data indicated that the extraction temperature affected total phenolic and antioxidant activity of both DPPH and FRAP, but did not affect the yield and total flavonoids. Extraction temperature of 55 °C had higher antioxidant activity with an IC50 DPPH value 362.52±7.10 ppm and FRAP value 70.30±10.01 μM equivalent to Fe (II)/g.

Author Biography

Amir Husni, Universitas Gadjah Mada
Fisheries 1

References

Ahmad AR, Juwita, Ratulangi SAD, Malik A. 2015. Penetapan kadar fenolik dan flavonoid total ekstrak metanol buah dan daun patikala (Etlingera elatior (Jack) R.M.SM). Pharmaceutical Sciences and Research. 2(1): 1-10.

Ahmad A, Nasrum M. 2013. Inhibitive enhancement of isoniasid treatment on Mycobacterium tuberculosis through triterpenoid carbocylic acid from red algae Euchema spinosum. International Journal of Pharma and Bio Sciences. 4(2): B231-B237.

Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. 2017. Phytochemicals: extraction, isolation, and identification of bioactive compounds from plant extracts. Plants. 6 (42): 1-23.

Demsi RP, Ruslan, Rahim EA, Ys H. 2019. Ekstraksi pektin pada kulit buah kluwih (Artocarpus camansiblanko) pada berbagai suhu dan konsentrasi asam sitrat. Kovalen: Jurnal Riset Kimia. 5(1): 100-108.

Dewi SR, Ulya N, Argo BD. 2018. Kandungan flavonoid dan aktivitas antioksidan ekstrak Pleurotus ostreatus. Rona Teknik Pertanian. 11(1): 1-11.

Fathmawati D, Abidin MRP, Roesyadi A. 2014. Studi kinetika pembentukan karaginan dari rumput laut. Jurnal Teknik Pomits. 3(1): 27-32.

Hanapi A, Fasya AG, Mardiyah U, Miftahurrahmah. 2013. Aktivitas antioksidan dan antibakteri ekstrak metanol alga merah Eucheuma spinosum dari perairan Wongsorejo Banyuwangi. Alchemy. 2(2): 126-137.

Husainah NU. 2020. Variasi suhu dan jenis pelarut pada ekstraksi daun tembakau kasturi inferior sebagai senyawa antibakteri. [Skripsi]. Jember (ID): Universitas Jember.

Hwang ES, Thi ND. 2014. Effects of extraction and processing methods on antioxidant compound contents and radical scavenging activities of laver (Porphyra tenera). Preventive Nutrition and Food Science. 19(1): 40-48.

Ibrahim AM, Yuniantha, Sriherfyna FH. 2015. Pengaruh suhu dan lama waktu ekstraksi terhadap sifat kimia dan fisik pada pembuatan minuman sari jahe merah (Zingiber officinale var. Rubrum) dengan kombinasi penambahan madu sebagai pemanis. Jurnal Pangan dan Agroindustri. 3(2): 530-541.

Jusuf E. 2010. Kandungan kuersetin dan pola proteomik varietas jambu batu (Psidium guajava L.) tumbuh liar dikawasan Cibinong, Bogor. Berita Biologi. 10(3): 401-415.

Kawiji, Khasanah LU, Utami R, Aryani NT. 2015. Ekstraksi maserasi oleoresin daun jeruk purut (Citrus hystrix DC): optimasi rendemen dan pengujian karakteristik mutu. Agritech. 35(2): 178-184.

Landjang EY, Momuat LI, Suryanto E. 2017. Efek pemanasan terhadap aktivitas antioksidan ekstrak empelur batang sagu baruk (Arenga microcarpha b.). Chemistry Progress. 10(1): 8-14.

Ling ALL, Yasir S, Matanjun P, Bakar MFA. 2015. Effect of different drying techniques on the phytochemical content and antioxidant activity of Kappaphycus alvarezii. Journal of Applied Phycology. 27: 1717-1723.

Maleta HS, Indrawati1 R, Limantara L, Brotosudarmo THP. 2018. Ragam metode ekstraksi karotenoid dari sumber tumbuhan dalam dekade terakhir (telaah literatur). Jurnal Rekayasa Kimia dan Lingkungan. 13(1): 40-50.

Neoh YY, Matanjun P, Lee JS. 2016. Comparative study of drying methods on chemical constituents of Malaysian red seaweed. Drying Technology. 34(14): 1745-1751.

Podungge A, Damongilala LJ, Mewengkang HW. 2018. Kandungan antioksidan pada rumput laut Eucheuma spinosum yang diekstrak dengan metanol dan etanol. Jurnal Media Teknologi Hasil Perikanan. 6(1): 197-201.

Sa’adah H, Nurhasnawati H. 2017. Perbandingan pelarut etanol dan air pada pembuatan ekstrak umbi bawang tiwai (Eleutherine americana Merr) menggunakan metode maserasi. Jurnal Ilmiah Manuntung. 1(2): 149-153.

Sangi M, Runtuwene MRJ, Simbala HEI, Makang VMA. 2008. Analisis fitokimia tumbuhan obat di Kabupaten Minahasa Utara. Chemistry Progress. 1(1): 47-53.

Santoso L, Nugraha YT. 2008. Pengendalian penyakit ice-iceuntuk meningkatkan produksirumput laut Indonesia. Jurnal Saintek Perikanan. 3(2): 37-43.

Sari BL, Susanti N, Sutanto. 2015. Skrining fitokimia dan aktivitas antioksidan fraksi etanol alga merah Eucheuma spinosumEucheuma spinosum. Pharmaceutical Sciences and Research. 2(2): 59-67.

Selawa W, Runtuwene MRJ, Citraningtyas G. 2013. Kandungan flavonoid dan kapasitas antioksidan total ekstrak etanol daun binahong (Anredera cordifolia (Ten) Steenis.). Pharmacon. 2(1): 18-23.

Setzer WN. 2008. Non-intercalative triterpenoid inhibitors of topoisomerase II: a molecular docking study. The Open Bioactive Compounds Journal. 1: 13-17.

Soehendro AW, Manuhara GJ, Nurhartadi E. 2015. Pengaruh suhu terhadap aktivitas antioksidan dan antimikrobia ekstrak biji melinjo (Gnetum gnemon l.) dengan pelarut etanol dan air. Jurnal Teknosains Pangan. 4(4): 15-24.

Syafitri NE, Bintang M, Falah S. 2014. Kandungan fitokimia, total fenol, dan total flavonoid ekstrak buah harendong (Melastoma affine D. Don). Current Biochemistry. 1(3): 105-115.

Virsa H, Ahmad AR, Sudir M. 2014. Uji aktivitas antioksidan ekstrak metanol bunga dan daun patikala (Etlingera elatior (Jack) R.M.Sm) menggunakan metode DPPH. Pharmaceutical Sciences and Research. 1(2): 86-93.

Wenjuan Q, Pan Z, Ma H. 2010. Extraction modeling and activities of antioxidants from pomegranate marc. Journal of Food Engineering. 99(1): 16–23.

Yanuarti R, Nurjanah, Anwar E, Hidayat T. 2017. Profil fenolik dan aktivitas antioksidan dari ekstrak rumput laut Turbinaria conoides dan Eucheuma cottonii. Jurnal Pengolahan Hasil Perikanan Indonesia. 20(2): 230-237.

Zainol MKM, Abdul-Hamid A, Abu-Bakar F, Pak-Dek S. 2009. Effect of different drying methods on the degradation of selected flavonoids in Centella asiatica. International Food Research Journal. 16: 531-537.

Published
2021-04-05