Harvesting and Separation Technique of Porphyridium cruentum Polysaccharide Using Ultrafiltration Membrane

  • Hasanah Hasanah Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Kampus IPB Dramaga, Jalan Agatis, Bogor 16680 Jawa Barat Telepon (0251) 8622909-8622906, Faks. (0251) 8622907
  • Iriani Setyaningsih Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Kampus IPB Dramaga, Jalan Agatis, Bogor 16680 Jawa Barat Telepon (0251) 8622909-8622906, Faks. (0251) 8622907
  • Uju Uju Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Kampus IPB Dramaga, Jalan Agatis, Bogor 16680 Jawa Barat Telepon (0251) 8622909-8622906, Faks. (0251) 8622907
Keywords: microalga, permeate, polysaccharide, separation, ultrafiltration

Abstract

Red microalga Porphyridium cruentum secreting polysaccharides into its medium culture. Harvesting
and separation of polysaccharide of P. cruentum usually use centrifugation and in large scale needs high
cost. The use of ultrafiltration membrane can be one of the alternatives for harvesting and separation of
P. cruentum polysaccharide. This study aimed to determine the characteristic of membrane and permeat
from harvesting and separation of P. cruentum polysaccharide using ultrafiltration. Research consisted of
four stages : membrane characterization, cultivation of P. cruentum, harvesting using 0.05 μm ultrafiltration
membrane, and polysaccharide separation using 0.01 μm ultrafiltration membrane. Characterization of
membrane permeability and internal resistance on ultrafiltration 0.05 μm dan 0.01 μm were 137.32 L/m2hbar
and 62.38 L/m2hbar and 0.01 barm2h/L and 0.02 barm2h/L, respectively. Harvesting using ultrafiltration 0.05
μm produced flux 131.37-94.75 L/m2h, biomass rejection 96% and permeate with OD (Optical Density) (0.01
± 0.00), viscosity (2.4 ± 0.17 cp), pH (8 ± 0.00), and salinity (42.37 ± 0.11 ‰). Separation of polysaccharide
using ultrafiltration 0.05 μm produced flux 58.11-51.53 L/m2h and permeate with viscosity (2.2 ± 0.30 cp),
pH (7.8 ± 0.01), and salinity (38.73 ± 0.05 ‰). Ultrafiltration process decreased OD, viscosity, and salinity
of permeate.

Published
2016-08-30