The Effect of Roselle (Hibiscus sabdariffa) Extract on Malondialdehyde Level in Rat Liver
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Issue
Vol. 16 No. 1 (2021)
Accepted
22 January 2021
Published
4 March 2021
Keywords:
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antioxidant, ethanol, malondialdehyde, roselle
Abstract
This study aimed to determine the efficacy of roselle flower extract in reducing the Malondialdehyde (MDA) level in rats after induction of 20% ethanol. This experimental study used a post-test design on 24 male white rats, Wistar strain which were grouped into six type of treatment. The K1 group was given daily aquadest only, K2 was given 20% ethanol. K3 was given 20% ethanol and vitamin C, K4 was given 20% ethanol and 250 mg/kgBW/day roselle flower extracts, K5 was given 20% ethanol and 500 mg/kgBW/day roselle flower extract, K6 was given 20% ethanol and 750 mg/kgBW/day roselle flower extract. Each group received treatment for 30 days. At the time of termination, the rat’s liver was collected and the liver’s MDA level was examined. One Way Anova test and the Post Hoc Tukey test were used for data analysis. There was a decrease in MDA levels (3.1578±0.37 ng/ml) in K4 compared to K2 as well as to K5 and K6 with higher extract concentrations. Thus, despite its benefit as antioxidant, excess of flavonoid compounds undergoing oxidation will produce a metabolite compound that can damage the endogenous antioxidant. Hence, 250 mg/kgBW/day roselle flower extract given daily can reduce MDA levels in mice induced with 20% alcohol.
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Sukardi S, Mulyarto AR, Safera W. 2007. Optimasi waktu ekstraksi terhadap kandungan tanin pada bubuk ekstrak daun jambu biji (Psidii Folium) serta biaya produksinya. Jurnal Teknologi Pertanian 8(2):88–94.
Syafitri NE, Bintang M, Falah S. 2014. Kandungan fitokimia, total fenol, dan total flavonoid ekstrak buah harendong (Melastoma affine D. Don). Current Biochemistry 1(3):105–115. https://doi.org/10.29244/cb.1.3.%25p.
Tritama TK. 2015. Konsumsi alkohol dan pengaruhnya terhadap kesehatan. Medical Journal of Lampung University 4(8):7–10.
Ulilalbab A, Wirjatmadi B, Adriani M. 2015. Ekstrak kelopak rosella merah mencegah kenaikan malondialdehid tikus wistar yang dipapar asap rokok. JIFI 13(2):215–220.
Werdhasari A. 2014. Peran antioksidan bagi kesehatan. Indonesian Jurnal Biotek Medisiana Indonesia 3(2):59–68.
Zakhari S. 2006. Overview: How is alcohol metabolized by the body? Alcohol Res Health 29(4):245–254.
Zuraida Z, Yerizel E, Anas E. 2015. Pengaruh pemberian ekstrak rosella (Hibiscus Sabdariffa Linn) terhadap kadar malondialdehid dan aktivitas katalase tikus yang terpapar karbon tetraklorida. Jurnal Kesehatan Andalas 4(3):795–802. https://doi.org/10.25077/jka.v4i3.366.
Aisiyah S, Harjanti R, Nopiyanti V. 2017. Pemanfaatan bunga rosella (Hibiscus sabdariffa L.) sebagai bahan alami untuk upaya preventif dan promotif kesehatan bagi masyarakat nusukan Banjarsari Surakarta. Abdimas Unwahas 2(1):5–8. http://dx.doi.org/10.31942/abd.v2i1.1786.
[AVMA] American Veterinary Medical Association. 2013. Guidelines for the Euthanasia of Animal: 2013 Edition. Schaumburg (US): AVMA.
Ayala A, Muñoz MF, Argüelles S. 2014. Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev 2014:360438. http://dx.doi.org/10.1155/2014/360438.
Christijanti W, Utami NR, Iswara A. 2011. Efek pemberian antioksidan vitamin c dan e terhadap kualitas spermatozoa tikus putih terpapar allethrin. Biosaintifika: Journal of Biology & Biology Education 2(1):18–26. https://doi.org/10.15294/biosaintifika.v2i1.1148.
Dianasari D, Fajrin A. 2015. Uji aktivitas antidiabetes ekstrak air kelopak bunga rosella (Hibiscus sabdariffa L.) pada tikus dengan metode induksi aloksan. Jurnal Farmasi Sains dan Terapan 2(1):1–4. https://doi.org/10.33508/jfst.v2i1.812.
Hayati Z, Winda Y, Karmil TF, Azmy A. 2012. Anti-bacterial Activity of Roselle Flowers Extract (Hibiscus sabdariffa Linn) in Inhibiting Bacterial Growth Methicillinr Esistant Staphylococcus aureus. Proceedings of The 2nd Annual International Conference Syiah Kuala University 2012 & The 8th IMT-GT Uninet Biosciences Conference (page 416–420), 22–24 November 2012, Banda Aceh (ID): Syiah Kuala University.
Hidayah N. 2011. Pengaruh Ketinggian Tempat Tumbuh Rosella dan Jenis Bahan Rosella terhadap Aktivitas Antioksidan Teh Kombucha Rosella [Undergraduate Thesis]. Malang: UIN Maulana Malik Ibrahim.
Fitria NL, Lyrawati D, Handaru M. 2015. Efek pemberian asam alfa lipoat terhadap kadar MDAdan gambaran histologi pada hati tikus wistar jantan dengan diabetes melitus tipe 1. Jurnal Kedokteran Brawijaya 28(3):170–176. https://doi.org/10.21776/ub.jkb.2015.028.03.2.
Harun I, Susanto H, Rosidi A. 2017. Pemberian tempe menurunkan kadar malondialdehyde (MDA) dan meningkatkan aktivitas enzim superoxide dismutase (SOD) pada tikus dengan aktivitas fisik tinggi. J Gizi Pangan 12(3):211–216. doi: 10.25182/jgp.2017.12.3.211-216.
Lieberman M, Peet A. 2018. MARKS' Basic Medical Biochemistry. 5th Edition. Philadelphia (US): Wolters Kluwer.
Lemmens KJA, Vrolijk MF, Bouwman FG, Van de Vijgh WJF, Bast A, Haenen GRMM. 2014. The minor structural difference between the antioxidants quercetin and 4’o-methylquercetin has a major impact on their selective thiol toxicity. Int J Mol Sci 15(5):7475–7484. https://doi.org/10.3390/ijms15057475.
Mardiah SR, Rahayu A, Ashadi RW, Sawarni H 2009. Budi Daya dan Pengolahan Rosela: Si Merah Segudang Manfaat. Jakarta (ID): Agromedia.
Nurnasari E, Khuluq AD. 2017. Potensi diversifikasi rosela herbal (Hibiscus Sabdariffa L.) untuk pangan dan kesehatan. Buletin Tanaman Tembakau, Serat & Minyak Industri 9(2):82–92.
Oktiarni D, Khasanah SN, Adfa M, Nesbah N, Angasa E. 2016. Pengaruh boraks, asam dan basa terhadap pergeseran panjang gelombang ekstrak air bunga rosella (Hibiscus sabdariffa Linn.) Gradien: Jurnal Ilmiah MIPA 12(2):1187–1191.
Pramita R, Widodo CS, Juswono UP. 2014, Pengaruh ekstrak kelopak rosella (Hibiscus sabdariffa L.) pada aktivitas SOD dan MDA mata tikus (Rattus wistar) yang dipapar radiasi gamma. Natural B 2(4):375–379.
Priska M, Peni N, Carvallo L, Ngapa YD. 2018. Review: Antosianin dan pemanfaatannya. Cakra Kimia: Indonesian E-Journal of Applied Chemistry 6(2):79–97.
[MoH RI] Ministry of Health Republic of Indonesia. 2018. Hasil Utama Riskesdas. https://kesmas.kemkes.go.id/assets/upload/dir_519d41d8cd98f00/files/Hasil-riskesdas-2018_1274.pdf.pdf [Accessed 30th January 2020].
Riskiyani S, Jannah M, Rahman A. 2015. Aspek sosial budaya konsumsi minuman beralkohol (Tuak) di Kabupaten Toraja Utara. Media Kesehatan Masyarakat Indonesia 11(2):76–85. http://dx.doi.org/10.30597/mkmi.v11i2.520.
Sukardi S, Mulyarto AR, Safera W. 2007. Optimasi waktu ekstraksi terhadap kandungan tanin pada bubuk ekstrak daun jambu biji (Psidii Folium) serta biaya produksinya. Jurnal Teknologi Pertanian 8(2):88–94.
Syafitri NE, Bintang M, Falah S. 2014. Kandungan fitokimia, total fenol, dan total flavonoid ekstrak buah harendong (Melastoma affine D. Don). Current Biochemistry 1(3):105–115. https://doi.org/10.29244/cb.1.3.%25p.
Tritama TK. 2015. Konsumsi alkohol dan pengaruhnya terhadap kesehatan. Medical Journal of Lampung University 4(8):7–10.
Ulilalbab A, Wirjatmadi B, Adriani M. 2015. Ekstrak kelopak rosella merah mencegah kenaikan malondialdehid tikus wistar yang dipapar asap rokok. JIFI 13(2):215–220.
Werdhasari A. 2014. Peran antioksidan bagi kesehatan. Indonesian Jurnal Biotek Medisiana Indonesia 3(2):59–68.
Zakhari S. 2006. Overview: How is alcohol metabolized by the body? Alcohol Res Health 29(4):245–254.
Zuraida Z, Yerizel E, Anas E. 2015. Pengaruh pemberian ekstrak rosella (Hibiscus Sabdariffa Linn) terhadap kadar malondialdehid dan aktivitas katalase tikus yang terpapar karbon tetraklorida. Jurnal Kesehatan Andalas 4(3):795–802. https://doi.org/10.25077/jka.v4i3.366.
Authors
AlyaniF. S., YuliantiR., & ThadeusM. S. (2021). The Effect of Roselle (Hibiscus sabdariffa) Extract on Malondialdehyde Level in Rat Liver. Jurnal Gizi Dan Pangan, 16(1), 57-62. https://doi.org/10.25182/jgp.2021.16.1.57-62
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