Pengaruh Pemberian Sinar Gamma terhadap Cabai Lokal Karo Terinfeksi Penyakit Virus Daun Keriting Kuning (Begomovirus)
Abstract
The Effect of Gamma Rays on Local Chilli Infected to Pepper Yellow Leaf Curl Virus Infection (Begomovirus)
Chili is a horticultural commodity that has a high economic value in Indonesia. The purple-stems Karo chili is one of local variety which has superiority in vegetative growth and production. However, it is susceptible against begomovirus infection. One of control attempt of Begomovirus is to create a new genotype resistant by using gamma ray irradiation. The research aimed to study the effect of gamma ray irradiation on begomovirus purple stems Karo local chili infecting seeds to obtain candidate of resistant genotype mutant generation 1 (M1) against begomovirus infection. The begomovirus infected seeds was irradiated by gamma rays at doses of 150, 200, 250 Gy, while untreated healthy and infected seeds were used as control. The variables observed including the disease incidence and disease severity as well as its area under disease progress curves (AUDPC) and virus detection on mutant first (M1) and mutan second generation (M2). The infected seeds irradiated by gamma ray at dose 150 Gy showed lowest disease intensity (incidence and severity) and AUDPC significantly in compare to other treatments on mutant plants first (M1) and second generation (M2). Based on the PCR detection, there are seven genotypes candidate mutants M2 which showed resistant against whitefly transmitted begomovirus.
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