@article{TALLEI_KOLONDAM_2015, title={DNA Barcoding of Sangihe Nutmeg (Myristica fragrans) using matK Gene}, volume={22}, url={https://jurnal.ipb.ac.id/index.php/hayati/article/view/9216}, DOI={10.4308/hjb.22.1.41}, abstractNote={<p><strong>Nutmeg (family: Myristicaceae) is a plant that originated from Banda islands and is widely cultivated in several places in the world. Secondary metabolites of this plant have a high value because of their benefits for the health, food, and beauty industries. This study aims at developing DNA barcode for nutmeg (<em>Myristica fragrans</em>) using standard recommended fragment of <em>mat</em>K (<em>maturase</em></strong> <strong>K) gene. Universal <em>mat</em>K primer pairs were used to amplify 889 bp DNA fragment. BLAST search from NCBI site showed that Sangihe nutmeg has 100% identity with</strong> <strong><em>Myristica fatua, M. maingayi,</em></strong><em> </em><strong>and</strong><em> <strong>M. globosa.</strong></em> <strong>It also has 3 nucleotides difference with <em>Rivola sebifera</em></strong> <strong>(identity 99.58%) and 4 nucleotides difference with <em>Knema laurina</em></strong> <strong>(identity 99.43%). It can be inferred from this study that single locus of <em>mat</em>K gene cannot be used to differentiate species in</strong> <strong><em>Myristica</em>; it can only be used to differentiate the genus level within family Myristicaceae.</strong></p&gt;}, number={1}, journal={HAYATI Journal of Biosciences}, author={TALLEITRINA EKAWATI and KOLONDAMBEIVY JONATHAN}, year={2015}, month={Jan.}, pages={41} }