@article{KHAERUNI_SUWANTO_TJAHJONO_SINAGA_1, title={Rapid Detection of Bacterial Pustule Disease on Soybean Employing PCR Technique with Specific Primers}, volume={14}, url={https://jurnal.ipb.ac.id/index.php/hayati/article/view/243}, DOI={10.4308/hjb.14.2.76}, abstractNote={<span style="font-family: Arial,Helvetica,Univers,Zurich BT; color: #003399;">A rapid polymerase chain reaction (PCR)-based procedure was developed for detection of <em>Xanthomonas axonopodis</em> pv. <em>glycines</em>, the causal agent of bacterial pustule disease on soybean. A set of primers was designed from partial sequence of the pathogenicity gene of X. <em>axonopodis</em> pv. <em>glycines</em> strain YR32. Specific PCR product of 490 base pairs was produced from strains of <em>X. axonopodis</em> pv. <em>glycines</em> originally from Indonesia as well as from Taiwan. No other pathovars and bacterial species among those tested showed amplification product under optimized PCR conditions. Shaking infected soybean leaves in phosphate buffer saline during six hours was proved to be an essential in order to increase cell number of the bacterial. The procedure was applicable and reliable for detecting of pathogens in infected plant materials. The procedure was proved to be more effective than that of conventional detection and could be of great help for monitoring of pustule bacterial disease in the soybean fields.<br /><br /> Key words: Xanthomonas axonopodis pv. glycines, bacterial pustule disease, rapid detection, PCR, specific primer </span&gt;}, number={2}, journal={HAYATI Journal of Biosciences}, author={KHAERUNI, ANDI and SUWANTO, ANTONIUS and TJAHJONO, BUDI and SINAGAMEITY SURADJI}, year={1}, month={1}, pages={76} }