@article{Khangembam_Sharma_Chakrabarti_2018, title={Diversity and Abundance of Ammonia-Oxidizing Bacteria and Archaea in a Freshwater Recirculating Aquaculture System}, volume={24}, url={https://jurnal.ipb.ac.id/index.php/hayati/article/view/20831}, DOI={10.4308/hjb.24.4.215}, abstractNote={<span>The role of ammonia-oxidizing bacteria<span> and archaea was evaluated using </span></span><em>amoA</em><span> gene in a freshwater recirculating system. Broken earthen pot pieces (BEP) were used as filter bed material. Five archaeal and four bacterial operational taxonomic units were retrieved from </span><em>amoA</em><span> genes. Shannon-Weiner and Simpson indices were higher in archaeal </span><em>amoA</em><span>sequence compared with the bacteria. Subtype diversity ratio and subtype diversity variance were 0.522 and 0.008, respectively, for archaea and 0.403 and 0.015, respectively, for bacteria. In archaea, 50% </span><em>amoA</em><span><span> sequences showed 99%–100% similarity with the known sequences of ammonia monooxygenase subunit A of uncultured archaeon </span>clones and thaumarchaeote. In bacteria, 84% sequences showed 99% similarity with </span><em>amoA</em><span>sequences of different uncultured bacterial clone and Nitrosomonadaceae. Absolute quantification showed that the abundance of archaea was 12-fold higher compared with bacteria. In this recirculating system, ammonia-oxidizing archaea and bacteria played a major role; BEP supported the growth of these ammonia-oxidizing microorganisms.</span&gt;}, number={4}, journal={HAYATI Journal of Biosciences}, author={KhangembamCherita Devi and SharmaJai Gopal and Chakrabarti, Rina}, year={2018}, month={Mar.}, pages={215} }