In silico Vaccine Design against Dengue Virus Type 2 Envelope Glycoprotein

  • Muhammad Adnan Gadjah Mada University, Faculty of Biology, Department of Tropical Biology, Daerah Istimewa Yogyakarta
  • Matin Nuhamunada Gadjah Mada University, Faculty of Biology, Department of Tropical Biology, Daerah Istimewa Yogyakarta
  • Lisna Hidayati Gadjah Mada University, Faculty of Biology, Department of Tropical Biology, Daerah Istimewa Yogyakarta
  • Nastiti Wijayanti Gadjah Mada University, Faculty of Biology, Department of Tropical Biology, Daerah Istimewa Yogyakarta

Abstract

Dengue fever is caused by the mosquito-borne virus termed (DENV). However, DENV-2 has been identified as the most prevalent amongst the Indonesian pediatric urban population, in contrast with the other four serotypes. Therefore, it is important to reduce severe infection risk by adopting preventive measures, including through vaccine development. The aim of this study, therefore is to use various in silico tools in the design of epitope-based peptide vaccines (T-cell and B-cell types), based on the DENV-2 envelope glycoprotein sequences available. Therefore, in silico methods were adopted in the analysis of the retrieved protein sequences. This technique was required to determine the most immunogenic protein, and is achieved through conservancy analysis, epitope identification, molecular simulation, and allergenicity assessment. Furthermore, B4XPM1, and KAWLVHRQW were identified from positions 204-212, while the 77 to 85 peptide region was considered the most potent T-cell and B-cell epitopes. The interaction between KAWLVHRQW and HLA-C*12:03 occurs with maximum population coverage, alongside high conservancy (96.98%) and binding affinity. These results indicated a potential for the designed epitopes to demonstrate high immunity against DENV-2.

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Published
2020-07-01
How to Cite
Adnan, M., Nuhamunada, M., Hidayati, L., & Wijayanti, N. (2020). In silico Vaccine Design against Dengue Virus Type 2 Envelope Glycoprotein. HAYATI Journal of Biosciences, 27(3), 228. https://doi.org/10.4308/hjb.27.3.228
Section
Articles