Screening of Endophytic Bacteria as Biocontrol Agents Against Bacteria Leaf Blight (Xanthomonas oryzae)

Rashidah Abd  Halim; Nor'Aishah  Hasan; Kogeethavani  Ramachandran

doi:10.4308/hjb.27.3.215

Screening of Endophytic Bacteria as Biocontrol Agents Against Bacteria Leaf Blight (Xanthomonas oryzae)

Rashidah Abd Halim Faculty of Applied Sciences, Universiti Teknologi MARA, Cawangan Negeri Sembilan, Kuala Pilah Negeri Sembilan
Nor'Aishah Hasan Faculty of Applied Sciences, Universiti Teknologi MARA, Cawangan Negeri Sembilan, Kuala Pilah Negeri Sembilan
Kogeethavani Ramachandran Laboratory of Plant Pathology, Rice and Industrial Crops Research Centre, MARDI Seberang Perai, Kepala Batas, Penang

DOI: https://doi.org/10.4308/hjb.27.3.215

Abstract

Bacterial leaf blight (BLB) is one of major threats in rice production as it can cause 100% yield loss. Concern on the environment and human health has led an attempt to replace existing methods of chemical control and avoid extensive use of bactericides by using endophytic bacteria. The present study was conducted to screen and characterize bacteria isolated from different sources that has potential as antagonistic bacteria against Xanthomonas oryzaepv. oryzae (Xoo), the causal agent of bacterial leaf blight of paddy. Two hundred and thirty-three endophytic bacteria were successfully isolated from roots and leaves from paddy field. Only 17 endophytic bacterial isolates showed positive antagonistic activity indicated by inhibition zone around bacterial colony against Xoo on nutrient agar plate with 2 endophytic isolates (BCA 3 and BCA 12) showed highest inhibitory effect with 35±0.00 mm in diameter. Molecular identification by 16S rRNA amplification successfully identified the antagonistic endophytic bacteria as Pseudomonas fluorescensand Geobacillus thermoparaffinivorans. Findings in this study revealed the biocontrol abilities of isolated endophytes as an excellent option to be used by agriculture sectors to have sustainable environment.

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Published

2020-07-01

How to Cite

HalimR. A., HasanN., & RamachandranK. (2020). Screening of Endophytic Bacteria as Biocontrol Agents Against Bacteria Leaf Blight (Xanthomonas oryzae). HAYATI Journal of Biosciences, 27(3), 215. https://doi.org/10.4308/hjb.27.3.215

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Vol. 27 No. 3 (2020): July 2020

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