Apoptotic Potential of Secretome from Interleukin-Induced Natural Killer Cells toward Breast Cancer Cell Line by Transwell Assay

  • Wahyu Widowati Faculty of Medicine, Maranatha Christian University, Bandung
  • Diana Krisanti Jasaputra Faculty of Medicine, Maranatha Christian University, Bandung
  • Teresa Liliana Wargasetia Faculty of Medicine, Maranatha Christian University, Bandung
  • The Fransiska Eltania Faculty of Medicine, Maranatha Christian University, Bandung
  • Alya Mardhotillah Azizah Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung
  • Mawar Subangkit Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, IPB University, Bogor
  • I Nyoman Ehrich Lister Faculty of Medicine, Universitas Prima Indonesia, Medan
  • Chrismis Novalinda Ginting Faculty of Medicine, Universitas Prima Indonesia, Medan
  • Ermi Girsang Faculty of Medicine, Universitas Prima Indonesia, Medan
  • Ahmad Faried Department of Neurosurgery and Stem Cell Working Group, Faculty of Medicine, Padjadjaran University, Bandung

Abstract

Breast cancer (BC) is the number one cause of deaths from cancer in women. Metastasis in BC is caused by immunosurveillance deficiency, including impairment of Natural Killer (NK) cell maturation, low NK activity, and decreasing cytotoxicity. This study was performed to improve activating receptors and cytotoxicity of NK cells using interleukin 15 (IL15) against BC cells. Human recombinant IL15 was used to induce NK cells. To evaluate the potential of IL15 in inducing NK cells, we measured the activating and inhibiting receptors (NKG2D, NKG2A), apoptotic potency of NK cells on BC cells (MCF7) using transwell assay. The IL15 inducer  on the NK cell were measured NKG2D, NKG2A gene expression with quantitative polymerase chain reaction (qPCR), (GzmB) secretion using ELISA, apoptotic gene expression of MCF7 using qPCR. IL15 increased NKG2D expression 4.01-9.13%, but IL15 could not affect toward NKG2A expression on NK cells. IL15-activated NK cells, inhibited BC cells proliferation, induced apoptotic BC cells 25.89-32.19%, induced apoptotic genes of BC cells bax, p53. IL15 increase NK activating receptor (NKG2D), inhibit BC cells proliferation, induce apoptotic percentage and induce apoptotic gene expression.

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Published
2020-07-01
How to Cite
Widowati, W., Jasaputra, D. K., Wargasetia, T. L., Eltania, T. F., Azizah, A. M., Subangkit, M., Lister, I. N. E., Ginting, C. N., Girsang, E., & Faried, A. (2020). Apoptotic Potential of Secretome from Interleukin-Induced Natural Killer Cells toward Breast Cancer Cell Line by Transwell Assay. HAYATI Journal of Biosciences, 27(3), 186. https://doi.org/10.4308/hjb.27.3.186
Section
Articles