Methylmercury Biosorption Activity by Methylmercury-resistant Lactic Acid Bacteria Isolated From West Sekotong, Indonesia
AbstractMethylmercury has been generally known as a toxic heavy metal for both human and environment. Bacterial-based bioremediation of heavy metal is suggested as an ecofriendly and low-cost bioremediation process. There was limited information regarding the role of lactic acid bacteria (LAB) as detoxification agent for methylmercury addressed for human body. West Sekotong, West Lombok, Indonesia, is one of the newly developed artisanal and small-scale gold mining site with high mercury contamination level. This present study was aimed to isolate the human origin methylmercury-resistant LAB and further evaluate their ability to absorb methylmercury. Methylmercury absorption assay was conducted in broth media. The remaining and absorbed methylmercury was measured using the gas chromatography flame ionization detector. A total of 56 methylmercury-resistant LAB isolates were isolated from 37 feces and 19 breast milk samples from 19 volunteers in West Sekotong. Of them, 10 isolates were further selected based on several basic probiotic characteristics and subjected to methylmercury removal assay. The selected isolates showed different methylmercury absorption ability ranged between 17.375 and 51.597 μg/g of wet mass of cell after incubated for 24 hours. Two isolates from feces showing the best removal activity were identified as Enterococcus durans and one isolates from breast milk as Enterococcus faecium based on the sequences of 16s rDNA.
HAYATI J Biosci article's license is CC-BY-NC. This license lets others distribute, remix, tweak, and build upon author's work, as long as they credit the original creation.
Authors who submit and publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal/publisher non exclusive publishing rights with the work simultaneously licensed under a https://creativecommons.org/
licenses/by-nc/4.0/Attributi on — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- Authors can still use their work commercially
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).