Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure

  • Margaretha Herawati Master Program in Biomedical Sciences, Faculty of Medicine University of Indonesia Department of Biochemistry, Faculty of Dentistry, Universitas Prof. Dr. Moestopo (Beragama), Jakarta
  • . Wardaya Indonesian Air Force Institute of Aviation Medicine (Lembaga Kesehatan Penerbangan dan Ruang Angkasa) Saryanto, Jakarta
  • Wawan Mulyawan Indonesian Air Force Institute of Aviation Medicine (Lembaga Kesehatan Penerbangan dan Ruang Angkasa) Saryanto, Jakarta
  • Fanny Septiani Farhan Department of Physiology, Faculty of Medicine Muhammadiyah University, Jakarta
  • Frans Ferdinal Department of Biochemistry and Molecular Biology, Faculty of Medicine Tarumanagara University, Jakarta
  • Sri Widia A. Jusman Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine University of Indonesia, Jakarta Department of Biochemistry and Molecular Biology, Faculty of Medicine University of Indonesia, Jakarta
  • Mohamad Sadikin Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine University of Indonesia, Jakarta Department of Biochemistry and Molecular Biology, Faculty of Medicine University of Indonesia, Jakarta
Keywords: intermittent hypobaric hypoxia, HIF-1a, Mb

Abstract

The aim of this study was to investigate the influence of intermittent hypobaric hypoxia on the expression hypoxia adaptation proteins, namely hypoxia inducibla factor-1a (HIF-1a) and myoglobin (Mb). Twenty five male Sprague-Dawley rats were exposed to intermittent hypobaric hypoxia in a hypobaric chamber in Indonesian Air Force Institute of Aviation Medicine, for 49.5 minutes at various low pressure, 1 week interval for 4 times (day 1, 8, 15 and 22). HIF-1α and Mb protein were measured with ELISA. mRNA expression of Mb was measured with one step real time RT-PCR. HIF-1α protein levels increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0437). mRNA expression and protein of Mb increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0283; 0.0170), and both are strongly correlated (Pearson, r = 0.6307). The heart of rats adapted to intermittent hypoxia conditions by upregulation the expression of HIF-1a and myoglobin and then both return to normal level.

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Published
2018-03-26
Section
Articles