Induction of Somatic Embryogenesis in Sengon (Falcataria moluccana) With Thidiazuron and Light Treatments

  • Ari Sunandar Plant Biology Graduate Program, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor 16680, West Java Biology Education Program, Faculty of Teaching Training and Education, Universitas Muhammadiyah Pontianak, Pontianak 78124, West Kalimantan
  • . Dorly Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor 16680, West Java
  • Ence Darmo Jaya Supena Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor 16680, West Java Research Center for Bioresources and Biotechnology, Bogor Agricultural University, Bogor 16680, West Java
Keywords: fast growing tree, secondary somatic embryogenesis, somatic embryo, woody legume

Abstract

Falcataria moluccana is important for reforestation and afforestation in Indonesia. However, epidemic of gall rust disease in F. moluccana plantations decreases its productivity. Genetic engineering is an alternative solution to against gall rust disease. Somatic embryogenesis is an efficient in vitro plant regeneration for successful plant improvement through genetic engineering. The objective of this study was to investigate the effect of thidiazuron and light treatments on the induction of somatic embryogenesis of Fmoluccana. The effects of thidiazuron concentration (5, 10 or 15 μM) and light (continuous light, 7 days of dark followed by light, or continuous dark) on the induction of somatic embryogenesis in leaf explants were assessed. The highest production of somatic embryos was obtained in 5 μM thidiazuron and dark treatments for 7 days followed by light in Murashige and Skoog medium supplemented with 1.2 g/L proline. Histological analysis in globular and cotyledon stages confirmed that cells had progressed to secondary somatic embryogenesis. This research needs more improvements to become a successful and efficient somatic embryogenesis method and as a potential method for successful plant improvement through genetic engineering in F. moluccana.

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Published
2018-03-26
Section
Articles