Expression Study of Banana Pathogenic Resistance Genes

Fenny M. Dwivany, Rizkita Rahmi Esyanti, Aksarani 'Sa Pratiwi, Herafi Zaskia

Abstract


Banana is one of the world's most important trade commodities. However, infection of banana pathogenic fungi (Fusarium oxysporum race 4) is one of the major causes of decreasing production in Indonesia. Genetic engineering has become an alternative way to control this problem by isolating genes that involved in plant defense mechanism against pathogens. Two of the important genes are API5 and ChiI1, each gene encodes apoptosisinhibitory protein and chitinase enzymes. The purpose of this study was to study the expression of API5 and ChiI1 genes as candidate pathogenic resistance genes. The amplified fragments were then cloned, sequenced, and confirmed with in silico studies. Based on sequence analysis, it is showed that partial API5 gene has putative transactivationdomain and ChiI1 has 9 chitinase family GH19 protein motifs. Data obtained from this study will contribute in banana genetic improvement.

Keywords


apoptosis inhibitor 5 (API5); Arabidopsis thaliana; chitinase (ChiI1); Musa acuminata; pathogen resistance

Full Text:

PDF


DOI: https://doi.org/10.4308/hjb.23.4.196

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Fenny M. Dwivany, Rizkita Rahmi Esyanti, Aksarani 'Sa Pratiwi, Herafi Zaskia

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.