Expression Study of LeGAPDH, LeACO1, LeACS1A, and LeACS2 in Tomato Fruit (Solanum lycopersicum)

  • Pijar Riza Anugerah Department of Biology, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung
  • Fenny Dwivany Department of Biology, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung
  • Rizkita Rahmi Esyanti Department of Biology, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung
Keywords: LeACS1A, LeACS2, LeACO1, LeGAPDH, ripening, tomato fruit

Abstract

Tomato is a climacteric fruit, which is characterized by ripening-related increase of respiration and elevated ethylene synthesis. Ethylene is the key hormone in ripening process of climacteric fruits. The objective of this research is to study the expression of three ethylene synthesis genes: LeACO1LeACS1ALeACS2, and a housekeeping gene LeGAPDH in ripening tomato fruit. Specific primers have been designed to amplify complementary DNA fragment of LeGAPDH (143 bp), LeACO1 (240 bp), LeACS1A (169 bp), and LeACS2 (148 bp) using polymerase chain reaction. Nucleotide BLAST results of the complementary DNA fragments show high similarity with LeGAPDH (NM_001247874.1), LeACO1 (NM_001247095.1), LeACS1A(NM_001246993.1), LeACS2 (NM_001247249.1), respectively. Expression study showed that LeACO1LeACS1ALeACS2, and LeGAPDH genes were expressed in ripening tomato fruit. Isolation methods, reference sequences, and primers used in this study can be used in future experiments to study expression of genes responsible for ethylene synthesis using quantitative polymerase chain reaction and to design better strategy for controlling fruit ripening in agroindustry.

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Published
2016-05-23
Section
Short Communication