Aktivitas Antibakteri Isolat Bakteri Endofit dari Tanaman Miana (<i>Coleus scutellariodes</i> [L.] Benth.) terhadap <i>Staphylococcus aureus</i> dan <i>Escherichia coli</i>

  • Dwi Endah Kusumawati Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
  • Fachriyan Hasmi Pasaribu Institut Pertanian Bogor
  • Maria Bintang Institut Pertanian Bogor

Abstract

Coleus scutellarioides [L.] Benth. known as medicinal plant which contains antidiarhea and antimicrobial compounds. Extraction of bioactive compound from plants is not efficient because it needs a large biomass, therefore the one of appropiate ways is using endophytic bacteria. The purpose of this research was to isolate and test of antibacterial activity of endophytic bacteria from Coleus scutellarioides [L.] Benth. against two pathogenic bacteria i.e Eschericia coli and Staphylococcus aureus. A total of 22 isolates of endophytic bacteria were obtained from Coleus scutellarioides [L.] Benth. Based on antibacterial activity test, 13 isolates of endophytic bacteria were able to inhibit the growth of E. coli and 15 isolates were able to inhibit S. aureus, whereas endophytic bacteria which can inhibit both types of pathogens are 10 isolates.

Author Biographies

Dwi Endah Kusumawati, Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
Sekolah Pascasarjana
Fachriyan Hasmi Pasaribu, Institut Pertanian Bogor
Mikrobiologi Medik, Fakultas Kedokteran Hewan
Maria Bintang, Institut Pertanian Bogor
Departemen Biokimia

References

Buchanan RE, Gibbons NE. 1974. Bergey’s Manual of Determination Bacteriology 8th ed. Baltimore: The Williams and Wilkins Co.

Castillo et al. 2002. Munumbicins, wide-spectrum antibiotics produced by Streptomyces NRRL 30562, endophytic on Kennedia nigriscans. Microbiology 148:2675–2685.

Desriani et al. 2013. Potential Endophytic Bacteria for Increasing Paddy Var Rojolele Productivity. IJASEIT 3(1):76-78.

Eliza, Munif A, Djatnika I, Widodo. 2007. Karakter fisiologis dan peranan antibiosis bakteri perakaran Graminae terhadap Fusarium dan pemacu pertumbuhan tanaman pisang. J Hort. 17:150-160.

Guan SH, Sattler I, Lin WH, Guo DA & Grabley S (2005) p-Aminoacetophenonic acids produced by a mangrove endophyte: Streptomyces griseus subspecies. J Nat Prod 68: 1198–1200.

Julianus et al. 2011. Tumbuhan Obat Tradisional di Sulawesi Utara. Ed ke-2. Manado: Balai Penelitian Kehutanan Manado.

Koomnok C, Teaumroong N, Rerkasem B, Lumyong S. 2007. Diazotroph endophytic bacteria in cultivated and wild rice in Thailand. Science Asia 33: 429-435.

Kumala S & Desi. 2009. Aktivitas antibakteri ekstrak daun iler (Coleus atropurpureus Benth) terhadap beberapa bakteri gram (+) dan bakteri gram (-). Jurnal Bahan Alam Indonesia. 7(1):12-14.

Lamb TG, Tonkyn DW, Kluepfel DA. 1996. Movement of Pseudomonas aureofaciens from the rhizosphere to aerial plant tissue. Can. J. Microbiol. 42:1112–1120.

Mano H & H Morisaki. 2008. Minireview: Endophytic bacteria in the rice plant. Microbes and Environments 23: 109-117.

Pal A, Chattopadhyay A, Paul AK. 2012. Diversity and Antimicrobial Spectrum of Endophytic Bacteria Isolated from Peaderi foetida L. Int J Curr Pharm Res. 4:123-127.

Rahmawati F. 2008. Isolasi dan Karakterisasi Senyawa Antibakteri Ekstrak Daun Miana (Coleus scuntellariodes L. Benth) [Tesis]. Bogor: Institut Pertanian Bogor.

Ryan RP, Germaine K, Franks A, Ryan DJ, Dowling DN. 2008. Minireview: Bacterial endophytes: recent development and application. FEMS Microbiol Lett 278: 1-9.

Simarmata R, Lekatompessy S, Sukiman H. 2007. Isolasi mikroba endofitik dati tanaman obat sambung nyawa (Gymura procumbens) dan analisis potensinya sebagai antimikroba. Berk Penel Hayati 13 : 85-90.

Strobel GA, Stierle A, Stierle D, Hess WM. 1993. Taxomyces andreanae a proposed new taxon for a bulbilliferous hyphomycete associated with Pacific yew Mycotaxon. 47:71–78.

Sundari D & Winarno MW. 1996. Pemanfaatan tumbuhan sebagai obat diare di Indonesia. Cermin dunia kedokteran 109: 25-32

Son R & Cheah YK. 2002. Preliminary Screening of Endophytic Fungi from Medical Plants in Malaysia for Antimicrobial and Antitumor Activity. Malaysian Journal of Medical Sciences 9(2): 23–33.

Tarabily K, Nassar AH, Sivasithamparam K. 2003. Promotion of Plant Growth By An Auxin Producing Isolate Of The Yeast Williopsis Saturnus Endophytic in Maize Roots. The Sixth UAE Universty Research Conference: 60-69.

Winarto WP. 2007. Tanaman Obat Indonesia untuk Pengobatan Herba. Ed ke-1. Jakarta: Karyasari Herba Media.

Published
2014-04-19
Section
Articles