Detection of Subclinical Mastitis in Dairy Cows using California Mastitis Test and Udder Pathogen

  • Evi Nur Qolbaini Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
  • I Made Artika Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
  • Dodi Safari Eijkman Institute for Molecular Biology, Diponegoro street 69
Keywords: mastitis, CMT, dairy cows

Abstract

Subclinical mastitis is an infection of the udder devoid of clinical symptoms, bacteria are one of the causes behind this disease. In the present study, we investigated subclinical mastitis in dairy cows using the California mastitis test (CMT) and udder pathogens from KUNAK (Kawasan Usaha Peternak Sapi Perah) Cibungbulang, Bogor, West Java, Indonesia. We randomly collected 102 milk samples from dairy farms in different stalls. We found that 87 out of the 102 (86 %) milk samples were positive for CMT test with level+1, level+2, and level+3 were 22 %, 45 %, and 33 % respectively. We also identified three different major bacterial groups: staphylococcus, streptococcus, and enterobacteria based on gram staining, oxidase test, and coagulase test. It can be concluded that the case of bovine subclinical mastitis in Kunak Bogor was very high and caused by various bacteria which infected cows.

References

Akers, R.M. 2002. Lactation and the Mammary Gland. Ames: Iowa State University Press, US

Barnum DA, Newbould FHS. 1961. The Use of California Mastitis Test for the Detection of Bovine Mastitis. Can Vet Jour. 2(3): 83-90

Bewley J, Arnold M. 2012. Recomended Milking Procedures for Maximum Milk Quality. University of Kentucky, US

Blowey R, Edmondson P. 2010. Mastitis Control in Dairy Herds 2nd Edition. London:CABI, UK

Bradley AJ, Leach KA, Breen JE, Green LE, Green MJ. 2007. Survey of the Incidence and Aetiology of Mastitis on Dairy Farms in England and Wales. Veterinary Record. 160:253-258

Cowan and Steels. 2003. Manual for the Identification of Medical Bacteria, third edition. UK:Cambridge University Press

Effendi MH. 2013. Angka Prevalensi Bovine Mastitis Beberapa Peternakan Sapi Perah di Jawa Timur [skripsi]. Surabaya:Universitas Airlangga

Harley JP, Prescott LM. 2002. Laboratory Exercise in Microbiology, fifth edition. USA: The McGraw-Hill Companies

Hidayat AP, Effendi AA, Fuad Y, Patyadi, Taguchi K, Sugiwaka T. 2002. Buku Petunjuk Teknologi Sapi Perah di Indonesia untuk Peternak: Kesehatan Pemerahan. Bandung:PT Sonysugema Pressindo, Indonesia

Jones GM. 2009. Understanding the Basics of Mastitis. Virginia: Virginia State University, US

Putri YS. 2012. Skrining dan Uji Aktivitas Enzim Protease Bakteri dari Limbah Rumah Pemotongan Hewan [skripsi]. Surabaya:Universitas Airlangga

Marlina. 2008. Identifikasi Bakteri Vibrio parahaemolitycus Dengan Metode Biolog Dan Deteksi Gen ToxR-nya Secara PCR. Jurnal Sains dan Teknologi Farmasi. 12 (1):11-17

Schroeder JW. 2012. Mastitis Control Programs: Bovine Mastitis and Milking Management. North Dakota:North Dakota State University, US

Seegers H, Fourichon C, Beaudeau F. 2003. Production Effects Related to Mastitis Economics in Dairy Cattle Herds. Vet Res, in press.

Sudarwanto, M. 1998. The IPB-I Reagent As An Alternative Tool To Detect Subclinical Mastitis. Media Veteriner, 5(1): 1-5

Surjowardojo P, Suyadi, Hakim L, Aulani’am. 2008. Ekspresi Produksi Susu pada Sapi Perah Mastitis. J. Ternak Tropika. 9 (2):1-11

Taponen S, Salmikivi L, Simojoki H, Koskinen MT, Pyörälä S. 2009. Real-time Polymerase Chain Reaction-based Identification of Bacteria in Milk Samples from Bovine Clinical Mastitis with no Growth in Conventional Culturing. J of Dairy Science. 26 (6):610-617

Zdanowicz M. 2002. Sand And Sawdust Bedding Affect Populations Of Coliforms, Klebsiella Spp. And Streptococcus Spp. On Teat Ends Of Dairy Cows Housed In Freestalls [thesis]. Colombia: University of British Columbia, Canada

Published
2014-08-05
Section
Articles