@article{Krissanti_Indrawati_Maryam_2021, title={Sintesis antigen AFB1-BSA dan konjugasi antibodi anti AFB1-BSA dengan nanopartikel emas sebagai pereaksi imunostrip}, volume={4}, url={https://jurnal.ipb.ac.id/index.php/arshivetlett/article/view/26137}, DOI={10.29244/avl.4.4.77-78}, abstractNote={<p>Aflatoksin B1 (AFB1) often contaminates a great variety of foods and animal feeds that will be dangerous if con-sumed by humans or animals.&nbsp; Rapid detection techniques that can be used in the field is really needed to monitor AFB1 contamination.&nbsp; The aims of this study were to perform synthesis of AFB1-BSA antigen and conjugation of antibody against AFB1-BSA to gold nanoparticle as immunostrip-test reagents. The AFB1-CMO was identified on TLC and AFB1-BSA was characterized using SDS PAGE. The AFB1-CMO formation indicated as a blue spot at 0.45 retention factor (Rf) on TLC and the AFB1-BSA antigen revealed as a single band protein at about 72 kDa molecular weight on the SDS PAGE.&nbsp; Conjugation of antibody against AFB1-BSA to gold nanoparticle resulted in the formation of red-dish purple compound which can be used for the detection of AFB1 on immunostrip. The optimum composition achieved in concentration of AFB1-BSA 1-1.5 mg/ml, IgG anti rabbit 0.1 mg/ml, and antibody against AFB1-BSA-gold nanoparticle conjugate in 0.5x0.5 cm² area characterized by the establishment of two reddish purple lines in the test and control zone.</p&gt;}, number={4}, journal={ARSHI Veterinary Letters}, author={Krissanti, Ita and Indrawati, Agustin and Maryam, Romsyah}, year={2021}, month={Jun.}, pages={77-78} }