Uji Daya Anthelmintik Ekstrak Etanol Daun Kumis Kucing (Orthosiphon aristatus (Blume) Miq) Terhadap Haemonchus contortus Secara In Vitro

Arif Rahman Jabal; Indria  Augustina; Trinovita Elsa; Francisca Diana  Alexandra; Debora  Jesika; Pransiska  Elma

Arif Rahman Jabal Depertemen Parasitologi, Fakultas Kedokteran, Universitas Palangka Raya
Indria Augustina Department of Parasitology, Faculty of Medicine, Palangka Raya University
Trinovita Elsa Department of Pharmacotherapy, Faculty of Medicine, Palangka Raya University
Francisca Diana Alexandra Department of Pharmacotherapy, Faculty of Medicine, Palangka Raya University
Debora Jesika Study Program of Medicine, Faculty of Medicine, Palangka Raya University
Pransiska Elma Study Program of Medicine, Faculty of Medicine, Palangka Raya University

Abstract

Haemonchosis is an infection caused by the Haemonchus contortus worm, a blood-sucking gastrointestinal parasite that can also infect humans. Orthosiphon aristatus (Blume) Miq leaves have been shown to have anthelmintic activity due to the presence of saponins, flavonoids, and tannins. This study aimed to determine the anthelmintic activity of Orthosiphon aristatus (Blume) Miq leaves ethanol extract against H. contortus. The infective H. contortus larvae were divided into six treatment groups: a 0.5% concentration group, a 1% concentration group, a 2% concentration group, a 4% concentration group, a positive control group (+), and a negative control group (-), each with four replicates. The study was conducted in six hours, with observations made at the 1st, 2nd, 4th, and 6th hours. The results showed that the death of the larvae increased with higher extract concentrations and longer exposure to the extract of Orthosiphon aristatus (Blume) Miq leaves. The time of death for infective H. contortus larvae given Orthosiphon aristatus (Blume) Miq leaves ethanol extract was at the 4th hour, and the effective dose of the extract as an anthelmintic was the concentration of 0.5%.

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