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Seleksi, Karakterisasi Morfologi, dan Identifikasi Aktinobakteri Penghasil Mananase Asal Hutan Tanah Jambi untuk Produksi Mananoligosakarida

  • Rahayu Wulan Sekolah Pascasarjana, Program Studi Mikrobiologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680
  • Rika Indri Astuti Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680
  • Yaya Rukayadi Departemen Sains Makanan, Fakultas Sains dan Teknologi Makanan, Universiti Putra Malaysia, Serdang- Salangor 43400, Malaysia
  • Sri Estuningsih Departemen Klinik Reproduksi Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680
  • Anja Meryandini Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680; Pusat Penelitian Sumberdaya Hayati & Bioteknologi (PPSHB)-LPPM IPB, Kampus IPB Darmga, Institut Pertanian Bogor, Bogor 16680

Abstract

 

As the world's largest producer of crude palm oil, Indonesia also produces high palm kernel cake (PKC) by-products. PKC has a high mannan content, so it can be used to produce prebiotic mannan-oligosaccharides (MOS). Enzymatic MOS production can be carried out using actinobacterial mannanase's microbiological approach. The HJ45B-1 isolate was the best isolate, with a peak enzyme production of 0,338 U/mL on the 10th day of incubation. The mannanase enzyme was stable in storage at 27°C. MOS production using 1% PKC substrate produced MOS with the best degree of polymerization (2-4) with incubation for 1-3 hours. Morphological characteristics and molecular identification based on the 16S rRNA gene indicated that the HJ45B-1 isolate was Streptomyces spp.

 

Keywords: actinobacteria, mannanase, mannan-oligosaccharides, palm kernel cake, Streptomyces

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Published
2022-04-14
How to Cite
WulanR., AstutiR. I., RukayadiY., EstuningsihS., & MeryandiniA. (2022). Seleksi, Karakterisasi Morfologi, dan Identifikasi Aktinobakteri Penghasil Mananase Asal Hutan Tanah Jambi untuk Produksi Mananoligosakarida. Jurnal Ilmu Pertanian Indonesia, 27(2), 279-286. https://doi.org/10.18343/jipi.27.2.279