Expression and Association of SCD Gene Polymorphisms and Fatty Acid Compositions in Chicken Cross
Stearoyl-CoA desaturase (SCD) is an integral membrane protein of endoplasmic reticulum (ER) that catalyzes the rate limiting step in the monounsaturated fatty acids from saturated fatty acids. Selection for fatty acids traits based on molecular marker assisted selection is needed to increase a value of chicken meat. This study was designed to analyze expression and associations of SCD gene polymorphisms with fatty acid traits in F2 kampung-broiler chicken cross. A total of 62 F2 kampung-broiler chicken cross (29 males and 33 females) were used in this study. Fatty acid traits were measured at 26 weeks of age. Samples were divided into two groups based on fatty acid traits (the highest and the lowest). Primers in exon 2 region were designed from the genomic chicken sequence. The SNP g.37284A>G was detected and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was then used to genotype. The expression of SCD gene was analyzed using quantitative real time PCR (qRT-PCR). The result showed that there were three genotypes (AA, AG, and GG) found in this study. The SCD|AciI polymorphism was significantly associated with palmitoleic acid (C16:1), fatty acids total and saturated fatty acid in 26 weeks old of F2 kampung-broiler chicken cross (P<0.05). The SCD gene was expressed for polyunsaturated fatty acids in liver tissue in two groups of chickens. In conclusion, the SCD gene could be a candidate gene that affects fatty acids traits in F2 kampung-broiler chicken cross.
Abubakar, E. Supriyatna, & Sutopo. 2014. Genotype distribution of local chicken crosbred in poultry breeding center Temanggung-Central Java. IRJES., 3:01-14.
Alexander, L.J., M.D. MacNeil, T.W. Geary, W.M. Snelling, D. C. Rule, & J.A. Scanga. 2007. Quantitative trait loci with additive effects on palatability and fatty acid composition of meat in a Wagyu-Limousin F2 population. Anim. Genet. 38: 506-513. https://doi.org/10.1111/j.1365-2052.2007.01643.x
AOAC. 2012. Official Methods of Analysis. 19th Ed. Association of Official Analytical Chemists, Washington, Arlington, USA.
Bohannon-Stewart, A., G. Kelley, B. Kimathi, S.H.K.V. Subramanya, J. Donkor, C. Darris, J. Tyus, A. Payne, S. Byers, D. Hui, S. Nahashon, F.C. Chen, M. Ivy, & X. Wang. 2014. Expression of potential regulatory gene in abdominal adipose tissue of broiler chickens during early development. Genet Res Int. 2014:1-10. https://doi.org/10.1155/2014/318304
Paton, C.M. & J.M. Ntambi. 2009. Biochemical and physiological function of stearoyl-CoA desaturase. Am. J. Physiol. Endocrinol. Metab. 297:38-37. https://doi.org/10.1152/ajpendo.90897.2008
Castro, L.F.C, J.M. Wilson. O. Goncalves, S. Galante-Oliveira, E. Rocha, & I. Cunha. 2011. The evolutionary history of the stearoyl-CoA desaturase gene family in vertebrates. BMC Evol. Biol. 11:132. https://doi.org/10.1186/1471-2148-11-132
Desert, C., M.J. Duclos, P. Blavy, F. Lecerf, F. Moreews, C. Klopp, M. Aubry, F. Herault, P.L. Roy, C. Berri, M. Douaire, C. Diot, & S. Lagarrigue. 2008. Transcriptome profiling of the feeding-to-fasting transition in chicken liver. BMC Genomics, 9:611. https://doi.org/10.1186/1471-2164-9-611
Dridi, S., M. Taouis, A. Gertler, E. Decuypere, & J. Buyse. 2007. The regulation of stearoyl-CoA desaturase gene expression in tissue specific in chickens. J. Endocrin. 192: 229-236. https://doi.org/10.1677/JOE-06-0070
Erkkilä, A., V.D.F. de Mello, U. Riserius, & D.E. Laaksonen. 2008. Dietary fatty acid and cardiovascular disease: An epidemiological approach. Prog. Lipid Res. 47:172-187. https://doi.org/10.1016/j.plipres.2008.01.004
Flowers, M.T & J.M. Ntambi. 2008. Role of stearoyl-coenzymeA desaturase in regulating lipid metabolism. Curr. Opin. Lipidol. 19:248–256. https://doi.org/10.1097/MOL.0b013e3282f9b54d
Gao, Y., R. Zhang, X. Hu, & N. Li. 2007. Application of genomic technologies to the improvement of meat quality of farm animals. Meat. Sci. 77: 36-45. https://doi.org/10.1016/j.meatsci.2007.03.026
Ghanem, H.M., A.I. Ateya, Y.Y. El Seady, S.M. Nasr, & N.A. El Kholy. 2016. Effect of breed, apoVLDL-II gene polymorphism, and metabolic biochemical markers on growth and body composition traits in commercial broiler breeds. Asian J Anim Vet Adv., 11: 548-555. https://doi.org/10.3923/ajava.2016.548.555
Grundy, S.M. 1994. Influence of stearic acid on cholesterol metabolism relative to other long-chain fatty acids. Am. J. Clin. Nutr. 60:986S-900S.
Gunawan, A. , K. Kaewmala, M.J. Uddin, M.U. Cinar, D. Tesfaye, C. Phatsara, E. Tholen, C. Looft, & K. Schellander. 2011. Association study and expression analysis of porcine ESR1 as a candidat gene for boar fertility and sperm quality. Anim Repro Sci. 128.11-21.
Heinemann, F.S. & J. Ozols. 2003. Stearoyl-CoA desaturase, a short lived protein of endoplasmic reticulum with multiple control mechanism. Prostaglandins Leukot. Essent. Fatty Acid. 68:122-133. https://doi.org/10.1016/S0952-3278(02)00262-4
Iida, K. & H. Akashi. 2000. A test of translational selection at ‘silent’ sites in the human genome: base composition comparisons in alternatively spliced genes. Gene 261: 93–102. https://doi.org/10.1016/S0378-1119(00)00482-0
Jiang, Z., J.J. Michal, D.J. Tobey, T.F. Daniels, D.C. Rule, & M.D. MacNeil. 2008. Significant associations of stearoyl-CoA desaturase (SCD1) gene with fat deposition and composition in skeletal muscle. Int. J. Biol. Sci. 4:345-351. https://doi.org/10.7150/ijbs.4.345
Lim, K.S., J.M. Kim, E.A. Lee, J.H. Choe, & K.C. Hong. 2013. A Candidate Single Nucleotide Polymorphism in the 3′ Untranslated Region of Stearoyl-CoA Desaturase Gene for Fatness Quality and the Gene Expression in Berkshire Pigs. Asian-Australas J Anim Sci. 28:151-157. https://doi.org/10.5713/ajas.14.0529
Maharani, D., D.W. Seo, N.R. Choi, S. Jin, M. Cahyadi, C. Jo, & J.H. Lee. 2013a. Association of FASN and SCD genes with fatty acid composition in broilers. CNU J. Agr. Sci. 40:215-220. https://doi.org/10.7744/cnujas.2013.40.3.215
Maharani, D., H. B. Park, J. B. Lee, C. K. Yoo, H. T. Lim, S. H. Han, S. S. Lee, M. S. Ko, I. C. Cho, & J. H. Lee. 2013b. Association of the gene encoding stearoyl-CoA desaturase (SCD) with fatty acid composition in an intercross population between Landrace and Korean native pigs. Mol. Biol. Rep. 40:73-80. https://doi.org/10.1007/s11033-012-2014-0
Nei, M. & S. Kumar. 2000. Moleculear Evolution and Phylogenetics. New York: Oxford University Press.
Nestel, P, P. Clifton, & M. Noakes. 1994. Effects of increasing dietary palmitoleic acid compared with palmitic and oleic acids on plasma lipids of hypercholesterolemic men. J. Lipid Res. 35:656-662.
Oh, D.Y., M.H. Jin, Y.S. Lee, J.J. Ha, B.Y. Kim, J.S. Yeo, & J.Y. Lee. 2013. Identification of Stearoyl-CoA Desaturase (SCD) Gene Interactions in Korean Native Cattle Based on the Multifactor-dimensionality Reduction Method. Asian-Australas. J. Anim. Sci. 26: 1218-1228. https://doi.org/10.5713/ajas.2013.13058
Ohsaki H., A. Thnaka, S. Hoashi, S. Sasazaki, K. Oyama, M. Taniguchi, F. Mukai, & H. Mannen. 2009. Effect of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herds. Anim Sci J. 80:225–232. https://doi.org/10.1111/j.1740-0929.2009.00638.x
Resnyk, C.W., W. Carre, Z. Wang, T.E. Porter, J. Simon, E.L.B. Duval, M.J. Duclos, S.E. Aggrey, & L.A. Cogburn. 2013. Transcriptional analysis of abdominal fat in genetically fat and lean chickens reveals adipokenesis, lipogenic genes and a link between hemostasis and leannes. BMC Genomics, 14:557. https://doi.org/10.1186/1471-2164-14-557
Sambrook, J., E.F. Fritschi, & T. Maniatis. 1989. Molecular cloning: a laboratorymanual. Cold Spring Harbor Laboratory Press, New York.
Schmittgen, T.D. & K.J. Livak. 2008. Analyzing realtime PCR data by the comparative CT method. Nat. Protoc., 3:1101-1108. https://doi.org/10.1038/nprot.2008.73
Sulandari, S. M.S.A. Zein, S. Paryanti & T. Sartika. 2007. Taksonomi dan Asal Usul Ayam Domestikasi. Keanekaragaman Sumber Daya Hayati Ayam Lokal Indonesia Manfaat dan Potensi. Pusat Penelitian Ekologi, Lembaga Ilmu Pengetahuan Indonesia, Bogor. Pp: 5-23.
Webb, E.C., & H.A. O’Neil. 2008. The animal fat paradox and meat quality. Meat Sci. 80:28-36.
Zhang, S., T. J. Knight, J. M. Reecy, & D. C. Beitz. 2008. DNA polymorphisms in bovine fatty acid synthase are associated with beef fatty acid composition. Anim. Genet. 39:62-70. https://doi.org/10.1016/j.meatsci.2008.05.029
Wu, X., X. Zhou, Q. Chang, Y. Zhang, Y. Li, L. Zhang, J. Huang, & B. Liang. 2013. The evolutionary pattern and the regulation of stearoyl-CoA desaturase genes. BioMed Res. Int. 2013: 1-12. https://doi.org/10.1155/2013/856521
Copyright (c) 2017 Media Peternakan
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.Authors submitting manuscripts should understand and agree that copyright of manuscripts published are held by Media Peternakan. The statement to release the copyright to Media Peternakan is stated in Form A. This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA) where Authors and Readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.