Karakteristik Fermentasi Rumen In vitro dengan Penambahan Sabun Kalsium Minyak Nabati pada Buffer yang Berbeda

  • S Suharti Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, Institut Pertanian Bogor
  • D N Aliyah Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, Institut Pertanian Bogor
  • S Suryahadi Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, Institut Pertanian Bogor

Abstract

Canola oil and flaxseed oil are vegetable oils as potential sources of unsaturated fatty acid that could improve the production and quality of beef meat.  However, the use of vegetable oils need to be protected to avoid biohydrogenation by rumen bacteria.  The research was aimed to analyse effect of flaxseed/canola oils calcium soap in the different buffer media on in vitro fermentation characteristic.  The experiment was conducted in a factorial randomized block design with 2 factors and 3 blocks based on rumen sampling time.  The first factor was sources of vegetable oils (canola and falxseed) and the second factor was type of buffer (Kajikawa and McDougall).  Variables observed were pH value, N-NH3 concentration, total volatile fatty acid (VFA), dry matter and organic matter digestibility.  Data obtained was analysed using Analysis of Variance and any significant different further tested using Duncan Multiple Range Test.  The results showed that there was no interaction between sources of diet and buffer.  Supplementation of canola and flaxseed oils protected by calcium soap at level 6% did not affect pH value, dry matter digestibility, rumen protozoa and total bacteria.  The use of different buffers affected pH value, dry matter digestibility, rumen protozoa and total bacteria.  It’s concluded that the used of flaxseed oil or canola oil calcium soap did not alter rumen fermentation and McDougall buffer could improve in vitro fermentation activity compare to Kajikawa buffer.

Keywords: biohydrogenation, calcium soap, canola oil, flaxseed oil, rumen fermentation

Downloads

Download data is not yet available.

References

Alexander G, Prabhakara Rao Z & Rama Prasad J. 2002. Effect of supplementing sheep with sunflower acid oil or its calcium soap on nutrient utilization. Asian-Aust J Anim Sci. 15 (9): 1288-1293

Bhatt RS, Karim SA, Sahoo A & Shinde AK. 2013. Growth performance of lambs fed diet supplemented with rice bran oil as such or as calcium soap. Asian-Aust J Anim Sci. 26 (6): 812-819

Carter JF. Potential of flaxseeds and flaxseed oil in baked goods and other products in human nutrition. Cereal Foods World. 1993; 38:754–759

Damron WS. 2006. Introduction to Animal Science. Ohio (USA): Prentice Hall

Dehority BA. 2004. Rumen Microbiology. Nottingham. (UK): Nottingham University Press

Enjalbert F, Combes S, Zened A & Meynadier A. 2017. Rumen microbiota and dietary fat: a mutual shaping. Journal of Applied Microbiology 123: 782—797

General Laboratory Procedures. 1966. Department of Dairy Science. University of Wisconsin, Madison

Gillis MH, Duckett SK, Sackmann JR, Realini CE, Keisler DH & Pringle TD. 2004. Effects of supplemental rumen protected conjugated linoleic acid or linoleic acid on feedlot performance, carcass quality, and leptin concentrations in beef cattle. J Anim Sci. 82:1419-1427

Hanim C, Yusiati LM & Alim S. 2009. Effect of saponin as defaunating agent on in vitro ruminal fermentation of forage and concentrate. J Indo Trop Anim Agric. 34 (4): 231-235

Hidayah N, Suharti S & Wiryawan KG. 2014. In vitro rumen fermentation of ration supplemented with protected vegetable oils. Med Pet. 37(2): 129-135

Hollander UT, Michael Eskin NA & Bertrand M. 2012. Canola and Rapeseed Production, Processing, Food Quality, and Nutrition. London (UK): CRC Press

Hristov AN, Ivan M & Mc.Allister TA. 2004. In vitro effects on individual fatty acids on protozoal numbers and on fermentation products in ruminal fluid from cattle fed a high concentrate, barley-based diet. J Anim Sci. 82 (9): 2693-2704

Jalc D, Certik M, Kundrikova K, & Namestkova P. 2007. Effect of unsaturated C18 fatty acids (oleic, linoleic, and α-linolenic acid) on ruminal fermentation and production of fatty acid isomers in anartificial rumen. J Vet Medic.52 (3): 87-94

Jenkins TC.1993. Lipid metabolism in the rumen. J Dairy Sci. 76 (12): 3851-3863

Jouany, J.P. 1991. Defaunation of the rumen. In: Rumen Microbial Metabolism and Ruminant Digestion. Paris( FR) : J.P Jouany (Ed.). INRA

Kajikawa H, Tajima K, Mitsumori M & Takenaka A. 2007. Effects of amino nitrogen on fermentation parameters by mixed ruminal microbes when energy or nitrogen is limited. J Anim Sci 78 (2):121-128

Kowalski ZM. 1997. Rumen fermentation,nutrient flow to the duodenum, and digestibility in bulls fed calcium soaps of rapeseed fatty acids and soya beanmeal coated with calcium soaps. Anim Feed sci Technol. 69 (4): 289 - 303

Machmuller A. 2006. Medium-chain fatty acids and their potential to reduce methanogenesis in domestic ruminants. Agric. Ecosysistem Environment. 112: (2-3):107-114

Mahesti G. 2009. Pemanfaatan protein pada domba lokal jantan dengan bobot badan dan aras pemberian pakan yang berbeda. Semarang (ID): Fakultas Peternakan Universitas Diponegoro

Maia MRG, Chaudhary LC, Bestwick CS, Richardson AJ, McKain N, Larson TR, Graham IA &Wallace RJ. 2010. Toxicity of unsaturated fatty acids to the biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens. J Microbiol. 10(52): 1-10

Mattjik AA & IM Sumertajaya. 2002. Perancangan Percobaan dengan Aplikasi SAS dan Minitab. Bogor (ID): Institut Pertanian Bogor Press

McDonald PR, Edwards A, Greenhalg JFD & Morgan CA. 2002. Animal Nutrition 6th Edition. New York: (US): Longman Scientific and Technical Co. Published in The United States with John Willey and Sons Inc

Owen FN & Zinn R. 1988. Protein Metabolism of Ruminant Animal Digestive Physiology and Nutrition. New Jersey. Reston Book Prentice Hall, Englewood Cliffs

Palmquist DL & Jenkins TC. 1980. Fat in lactation rations: review. J Dairy Sci. 63 (1):1-14

Pantoja J, Firkins JL, Estridge ML & Hull BL.1994. Effect of fat saturation and source of fiber an site of nutrient digestion and milk production by lactating dairy cow. J Dairy Sci. 77 (8):2341-2356

Perez JM Martinez, Robles-Perez D, Benavides J, Moran L, Andres S, Giraldez F J, Rojo-Vazquez F A & Martinez-Valladares M. 2014. Effect of dietary supplementation with flaxseed oil or vitamin E on sheep experimentally infected with Fasciola hepatica. Res Vet Sci. 97 (1): 71–79

Prawirokusumo S. 1993. Ilmu Gizi Komparatif. Yogyakarta (ID): Fakultas Ekonomi dan Bisnis, Universitas Gajah Mada

Prieto N, Dugan MER, Lopez-Campos O, McAllister TA, Aalhus JL & Uttaro B. 2012. Near infrared reflectance spectroscopy predicts the content of polyunsaturated fatty acids and biohydrogenation products in the subcutaneous fat of beef cows fed flaxseed. Meat Sci. 90 (1): 43–51

Sakinah D. 2005. Kajian suplementasi probiotik bermineral terhadap produksi VFA, NH3, dan kecernaan zat makanan pada domba. Fakultas Peternakan, Bogor (ID): Institut Pertanian Bogor.

Sayuti N. 1989. Ruminologi. Padang (ID):Fakultas Peternakan Universitas Andalas

Sirohi SK, Malik R & Walli TK. 2001. Development and evaluation of protected fat in wheat straw based total mixed ration. Asian-Aust J Anim Sci. 14(10): 1405-1408

Soeparno. 1998. Ilmu dan Teknologi Daging. Gadjah Mada University Press, Yogyakarta

Tilley JMA & Terry RA. 1963. A two-stage technique for the in vitro digestion of forage crops. J Br Grassland Soc.18: 104-111

Woolford MK. 1984. The Silage Fermentation. New York (UK): Marcel dekker Inc

Published
2019-01-22
How to Cite
SuhartiS., AliyahD. N., & SuryahadiS. (2019). Karakteristik Fermentasi Rumen In vitro dengan Penambahan Sabun Kalsium Minyak Nabati pada Buffer yang Berbeda. Jurnal Ilmu Nutrisi Dan Teknologi Pakan, 16(3), 56-64. https://doi.org/10.29244/jintp.16.3.56-64