Aplikasi Gelombang Ultrasonik untuk Meningkatkan Rendemen Ekstraksi dan Efektivitas Antioksi dan Kulit Manggis

Mar'atus Sholihah, Usman Ahmad, I Wayan Budiastra

Abstract


Abstract

Maceration is one of the common extraction methods used to obtain antioxidant of mangosteen rind. However, this method time consuming and produce low extraction yield. Therefore, it needs other methods. One of them is ultrasonic-assisted extraction (UAE). The aim of the research is to observe the effect of UAE method on the increasing of extraction yield and the effectiveness of antioxidant from mangosteen rind. Three level of excitation time (15, 30, 45 minutes) and amplitude of ultrasonic wave (35, 50, 65%) were tested on UAE. As the control was maceration method at 35 oC for 7 hours. The result showed that extraction yield, antioxidant activity (IC50) and total anthocyanin content (TAC) of all ultrasonic treatments were significantly different from that of control. The optimum condition of UAE was obtained from amplitude of 65% and excitation time of 45 minutes resulting 6.71% of extraction yield, IC50 4.93 ppm and TAC 558.76 ppm. UAE can enhance the effectiveness antioxidant and reduce extraction time from mangosteen rind.

 

Abstrak

Maserasi adalah salah satu metode ekstraksi yang umum digunakan untuk mendapatkan antioksidan kulit manggis. Metode ini membutuhkan waktu yang cukup lama dan menghasilkan rendemen yang rendah. Oleh karena itu, diperlukan metode ekstraksi yang lebih cepat salah satunya dengan ultrasonic-assisted extraction (UAE). UAE adalah metode ekstraksi menggunakan bantuan ultrasonik. Tujuan dari penelitian ini adalah mengkaji pengaruh metode ultrasonik untuk peningkatan rendemen dan efektivitas antioksidan dari kulit manggis. Tiga level waktu eksitasi (15, 30, 45 menit) dan amplitudo (35, 50, 65%) diuji pada ekstraksi berbantu ultrasonik. Maserasi pada suhu 35oC selama 7 jam digunakan sebagai kontrol. Hasil penelitian menunjukkan bahwa rendemen, aktivitas antioksidan (IC50) dan kadar antosianin total (TAC) dari ekstraksi berbantu ultrasonik berbeda nyata terhadap kontrol. Kombinasi perlakuan terbaik dari ekstraksi berbantu ultrasonik adalah menggunakan amplitudo 65% dan waktu eksitasi 45 menit yang menghasilkan rendemen 6.71%, aktivitas antioksidan IC50 4.93 ppm dan kadar antosianin total 558.76 ppm. Ekstraksi berbantu ultrasonik mampu meningkat rendemen, efektivitas antioksidan dan mengurangi waktu ekstraksi kulit manggis.


Keywords


extraction, mangosteen rind, ultrasonic

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