Potency of Biological Agent Trichoderma harzianum and Gliocladium sp. on Pathogenic Fungi Botryodiplodia sp. causes Dieback Disease of Jabon (Anthocephalus cadamba (Roxb.) Miq.) POTENSI Trichoderma harzianum DAN Gliocladium sp. SEBAGAI AGENS HAYATI

Abstract

Botryodiplodia sp. is causes dieback disease of jabon (Anthocephalus cadamba (Roxb.) Miq). Dieback disease causes a decrease in the quality and economic value of jabon seedlings in the nursery. Research on control of the disease is still rare. Control of the disease is divided into three ways, namely chemical, physical, and biological. One biological control can be biological agents. Biological agents are used in this study is Trichoderma harzianum and Gliocladium sp. on in vitro test. The test results showed that T. harzianum antagonism with the direct method able to inhibit the growth of Botryodiplodia sp. with the average 52.53% and 35.99% respectively on PDA and Czapex Agar, while Gliocladium sp. able to inhibit the growth of Botryodiplodia sp. Average 46.46% and 28.51% respectively on the PDA and Czapex Agar for 7 days of observation. Results of antagonist test with indirect methods showed that the filtrate of T. harzianum and Gliocladium sp. has the ability to inhibit the growth of Botryodiplodia sp. at 13.42% and 10.25% PDB media significantly different from controls. T. harzianum and Gliocladium sp. have ability to inhibit the growth of Botryodiplodia sp. greatly.

Key words : Botryodiplodia sp., Gliocladium sp., in vitro test, Trichoderma harzianum