Karakteristik dan Aktivitas Antioksidan Rumput Laut Gracilaria sp. Asal Banten

Characteristics and Antioxidant Activities of Gracilaria sp. Seaweed from Banten

  • Sri Purwaningsih Departemen Teknologi Hasil Perairan, FPIK IPB University
  • Eka Deskawati IPB University
Keywords: antioxidant, bioactive compound, heavy metal, Gracilaria sp., proximate

Abstract

The Gracilaria sp. seaweed is often used as a source of commercial agar, however in recent years the price of this seaweed has dropped dramatically. Red seaweed has many health benefits. This study was aimed to determine proximate contains, heavy metal, total phenol and total flavonoid, and antioxidant activity of the seaweed Gracilaria sp. Extraction using the maceration method with ethanol, ethyl acetate, and n-hexane solvents with a ratio of 1:6 had a significant effect (α=0.05) on the antioxidant activity.The highest antioxidant activity was observed fromthe ethanolic extract with IC50 =22.15±1.63 μg/mL (very strong). Qualitative testing of the bioactive components of the ethanol extract of Gracilaria sp. found alkaloids, phenols, saponins, flavonoids, triterpenoids. Tests for total flavonoids and total phenols were carried out using a UV-Vis spectrophotometer obtaining 21.78±0.32 mg QE/g and 124±2.13 mg GAE/g respectively.

References

Afiah RN , Supartono W , Suwondo E. 2019. Potential of heavy metal contamination in cultivated red seaweed (Gracilaria sp. and Eucheuma cottonii) from coastal area of Java, Indonesia. IOP Conf. Series: Earth and Environmental Science 365 (2019) 012024 IOP Publishing doi:10.1088/1755-1315/365/1/012024
Amaranggana L, Wathoni N. 2017. Manfaat alga merah (Rhodopyta) sebagai sumber obat dari bahan alam. Majalah Farmasetika. 2(1): 16-19.
[AOAC] Association of Official Analytical Chemyst. 1995. Official Method of Analysis of The Association of Official Analytical of Chemist. Arlington, Virginia, USA : Published by The Association of Official Analytical Chemist. Inc.
[AOAC] Association of Official Analytical Chemyst. 2005. Official Method of Analysis (18 Edn). Arlington, Virginia, USA : Published by The Association of Official Analytical Chemist. Inc.
Bleakley, S., & Hayes, M. (2017). Algal Proteins: Extraction, application, and challenges concerning production. Foods, 6(5), 33. https://doi.org/10.3390/foods6050033.
[BSN] Badan Standardisasi Nasional. 2014. Cara uji kimia Bagian 5: Penentuan kadar logam berat timbal (Hg) dan kadnium (Cd) pada produk perikanan: SNI 2354.5-2011. Jakarta: Badan Standarisasi Nasional.
[BSN] Badan Standardisasi Nasional. 1998. Cara uji cemaran arsen dalam makanan: SNI 01- 4866-1998. Jakarta: Badan Standardisasi Nasional.
Chakraborty K dan Joseph D. 2016. Antioxidant potential and phenolic compounds of brown seaweeds Turbinaria conoides and Turbinaria ornata (class: phaeophyceae). Journal of Aquatic Food Product Technology. 25(8):1249–1265
Choi HG, Kim YS, Kim JH, Lee SJ, Park EJ, Ryu J, Nam KW. 2006. Effects of temperature and salinity on the growth of Gracilaria verrucosa and Gracilaria chorda, with the potential for mariculture in Korea. Journal of Applied Phycology 18: 269–277.
Coura, C. O., de Araújo, I. W. F., Vanderlei, E. S. O., Rodrigues, J. A. G., Quinderé, A. L. G., Fontes, B. P., et al. (2012). Antinociceptive and anti-inflammatory activities of sulphated polysaccharides from the red seaweed Gracilaria cornea. Basic and Clinical Pharmacology and Toxicology, 110(4), 335–341. https://doi.org/10.1111/j.1742- 7843.2011.00811.x
[FAO] Food and Agriculture Organization. 2014. FAO World Aquaculture. Rome(IT): Silicon Valley.
Hall AC, Fairclough AC, Mahadevan K, Paxman JR. 2012. Ascophyllum nodosum enriched bread reduces subsequent energy intake with no effect on postprandial glucose and cholesterol in healthy, overweight males. Journal Appetite. 58: 379-386.
Hasan MR, Rejeki S, Wisnu R. 2015. Pengaruh bobot awal yang berbeda terhadap pertumbuhan Gracilaria sp. yang dibudidayakan dengan metode longline di perairan tambak terabrasi Desa Kaliwlingi Kabupaten Brebes. Journal of Aquaculture Management and Technology. 4(2): 92-99.
[KKP] Kementerian Kelautan dan Perikanan. 2014. Siaran Pers: Pacu produksi rumput laut, kkp kembangkan pola budidaya polikultur. Jakarta(ID): Kementerian Kelautan dan Perikanan.
La Barre, S.; Potin, P.; Leblanc, C.; Delage, L. The halogenated metabolism of brown algae (Phaeophyta), its biological importance and its environmental significance. Mar. Drugs 2010, 8, 988–1010.
Mattjik AA, Sumertajaya M. 2013. Perancangan Percobaan dengan Aplikasi SAS dan Minitab. Bogor (ID): IPB Press.
Mahardika RG, Roanisca O. 2018. Aktivitas antioksidan dan fitokimia dari ekstrak etil asetat pucuk idat (Cratoxylum glaucum). Indonesian Journal Of Chemical Research. 5(2): 69-74.
Matanjun P, Mohamed S, Mustapha NM, Muhammad K, Ming CH. 2008 Antioxidant activities and phenolic content of eight species of seaweed fromNorth Borneo. Journal Applied Phycology. 20:367–373.
Meenakshi SDM, Gnanambigai ST, Mozhi M, Arumugam T, Balasubramanian. 2009. Total flavonoid and in vitro antioxidant activity of two seaweed of rameshwaram coast. Global Journal of Pharmacology. 3(2):59–62.
Munandar A, Surilayani D , Haryati S , Sumantri MH , Aditia R P , dan Pratama G. 2019. Characterization flour of two seaweeds (Gracilaria spp. and Kappaphycus alvarezii) for reducing consumption of wheat flour in Indonesia. IOP Conf. Series: Earth and Environmental Science 383 (2019) 012009. IOP Publishing doi:10.1088/1755-1315/383/1/012009.
Rajauria G, Foley B, Abu–Ghannam N. 2016. Identification and characterization ophenolic antioxidant compounds from brown irish seaweed himanthalia elongata using LC–DAD–ESI–MS/MS. Journal Innovative Food Science and Emerging Technologies. 37:261–268.
Redha A. 2010. Flavonoid: Struktur, sifat antioksidan dan peranannya dalam system biologis. Jurnal Berlian. 9 (2): 196-202.
Sanger G. 2010. Kandungan fosfor minuman sari rumput laut (Eucheuma cottonii). Pacific Journal. 1(5):792–795.
Sinulingga M, Darmanti S. 2006. Kemampuan untuk mengikat air oleh tanah pasir yang dibutuhkan oleh Gracilaria verrucosa. Jurnal Perikanan dan Kelautan. 6(4): 56-72.
Soelistyowati GDT, Murni IAAD, Wiyoto. 2014. Morfologi Gracilaria spp. yang dibudidaya di tambak desa pantai sederhana, Muara Gembong. Jurnal Akuakultur Indonesia. 13(1): 94–104.
Son YR, Choi EH, Kim GT, Park TS, Shim SM. 2015. Bioefficacy of graviola leaf extracts in scavenging free radicals and upregulating antioxidant genes. Journal Food and Fungtion Royal Society of Chemistry. 7(2):61–71.
Talsha Irzaputri A, Yoifah Rizka, Sarianoferni. Fakultas Kedokteran Gigi Universitas Hang Tuah Yoifah Rizka, Department of Periodontology,Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5912191
Wan–Ibrahim WI, Sidik K, Kuppusamy UR. 2010. A high antioxidant level in edible plants is associated with genotoxic properties. Food Chemistry. 122:1139–1144.
Xiong Y, Yuan C, Chen R, Dawson TM, Dawson VL. 2010. Preparation and biological activity saponin Ophiogon japonicas. African Journal of Pharmacy and Pharmacology. 6 (2): 1964-1970.
Zhu, Yan Q, Huang Y, Chen ZY. 2000. Insteractions between falvonoids and α-tocopherol in human low density lipoprotein. The Journal of Nutritional Biochemistry. 11 (1): 14-21.
Published
2021-01-02