Cytotoxic Activity of Alpinumisoflavone from Erythrina poeppigiana (Leguminosae) Against Colon Cancer (WiDr), Cervical Cancer (Hela), and Hepatoma Cancer (HepG2) Cells

Abstract

Cancer is the second cause of death after cardiovascular diseases in the world. Anticancer prevention used can cause undesirable things. Flavonoids are secondary metabolites derived from natural products that are useful for anticancer treatment. This study was performed to observe the cytotoxic activity of alpinumisoflavone from Erythrina poeppigiana, toward cervical cancer (Hela), colon cancer (WiDr), and hepatoma cancer (HepG₂) cells. The cytotoxic activity of alpinumisoflavone was tested using (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide) assay. The percentage of cell mortality was calculated and the IC₅₀ was calculated using probit analysis. The result shown that alpinumisoflavone has antiproliferative effect to colon cancer (WiDr), cervical cancer (Hela), and hepatoma cancer (HepG₂) cells with the value of IC₅₀ are 5.63, 7.18, and 18.08 µg/ml, respectively. Based on the value of IC₅₀ alpinumisoflavone is very cytotoxic to colon cancer WiDr cell.