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Validasi Metode Analisis Kolesterol dalam Telur dengan HPLC-ELSD

  • Hanifah Nuryani Lioe
  • Tika Setianingrum
  • Ririn Anggraeni
Keywords: cholesterol analysis, egg, HPLC-ELSD, method validation

Abstract

A method using high-performance liquid chomatography (HPLC) coupled with an evaporative light-scattering detector (ELSD) for the determination of cholesterol in egg was validated. A silica column and a binary mixture of hexane and isopropanol (90:10) as a mobile phase were used to separate cholesterol. Cholesterol was detected at 1.5 min using cholesterol standard and HPLC-ELSD condition: evaporation temperature 50 ºC, air pressure 2.2 bars, and flow rate of mobile phase 2 mL/min. A method linearity for the cholesterol analysis in egg as a sample matrix was obtained at a range of 50 to 3000 µg/g sample, with R2>0.990. Instrument detection limit and limit of quantitation were determined at 1.07 and 3.56 µg/mL, respectively. Recovery test results by spiking cholesterol standard in egg sample at low, medium, and high concentrations (50, 250 and 3000 µg/g ) were 122.13, 108.23, and 44.71%, respectively. Their corresponding repeatability values were 5.26, 4.29, and 10.11%. Method detection limit and intralab reproducibility (to analyze a sample) were observed at 2.30 µg/g and 0.04%. The method is valid for cholesterol analysis in egg at low and medium concentrations.

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Author Biographies

Hanifah Nuryani Lioe
Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Tika Setianingrum
Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Ririn Anggraeni
Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680

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Published
2014-08-24
How to Cite
Lioe, H. N., Setianingrum, T., & Anggraeni, R. (2014). Validasi Metode Analisis Kolesterol dalam Telur dengan HPLC-ELSD. Jurnal Ilmu Pertanian Indonesia, 18(3), 178-185. Retrieved from http://jurnal.ipb.ac.id/index.php/JIPI/article/view/8395

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