Penggandaan Skala Produksi Bioinsektisida Bacillus thuringiensis var. israelensis untuk Membasmi Jentik Nyamuk Aedes aegypti

Mulyorini Rahayuningsih, Khaswar Syamsu, Abdul Aziz Darwis, Rini Purnawati

Abstract


The objective of this research is to study the scaling up of bionsecticide production from Bacillus thuringiensisvar. israelensisusing onggok (a cassava by-product) as a carbon source. The insecticide produced was used to eradicate Aedes aegypti larvae. The product was a crystal protein produced during bacterial sporulation. Scaling up from laboratory to pilot plant scale was done using two methods, i.e. constant agitation power per unit volume (Pg/V) and constant oxygen transfer coefficient (kLa). The results showed that yield of product per substrate (Yp/s) of Pg/V based product with the value of 3.52 ± 0.02 spora per gram substrates was higher than Yp/s of  kLa based product with the value of 2.96 spora per gram substrate.  Logarithmic value of viable spore count (log of VSC) was also higher, i.e. 7.23 ± 0.30 for Pg/V based product as compared to 7.17 ± 0.20 for kLa based product. Substrate efficiency was also higher in Pg/V based (92.47%) than kLa based (64.87%). LC50 of Pg/V based product was lower (0.49 μg/ml) meaning that it was more toxic than kLa based product (0.62 μg/ml). Amino acid content of Pg/V based product was also higher than kLa based product. Constant Pg/V method was suggested as a based on the scaling up of bioinsecticide production of B. thuringiensis israe/ensison industrial scale.

Keywords: bioinsecticide, Bacillus thuringiensisvar. israelensis, kLa, Pg/V, LC50, viable spore count


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