Keragaman Morfometrik dan Genetik Gen COI Belut Sawah (Monopterus albus) Asal Empat Populasi di Jawa Barat

  • Lella Herdiana
  • Muhammad Mukhlis Kamal
  • Nurlisa Alias Butet
  • Ridwan Affandi
Keywords: genetic diversity, Monopterus albus, morphometric diversity, West Java

Abstract

Swamp eel (Monopterus albus) is an economically important freshwater fish which has the potential to sustainable development. This species is considered as one of the taxonomically problematic spesies due to its complex characters and similar morphology to Monopterus cuchia and Monopterus javanensis that often lead to taxonomic dilemmas. Taxonomic certainty is an essential basis information in sustainable fisheries management, studies on morphometric characters, and genetic variability is a method which commonly used for species or stock identification. This study was aimed to validate the taxonomy certainty and evaluate the kinship of swamp eel from four populations (Indramayu, Subang, Tasikmalaya, and Garut distrik) in West Java. Morphometric analysis was conducted by measurement of 19 characters, whereas genetic analysis was conducted using PCR-Sequencing method of COI gene. The results from stepwise discriminant analysis showed that 7 morphometric characters ratio were powerful to classified the populations and the first two discriminant functions accounted 84.2% of the total variability. Genetic diversity indicated by alignment 14 sekuen of COI gene showed there were 11 haplotypes from 109 variable sites. Results of cluster and phylogenetic tree analysis indicated that swamp eel from four research populations were grouped into two major clusters. The first cluster consists of Indramayu and Subang population, the second cluster consists of Tasikmalaya and Garut population. This cluster is an evidence that swamp eel from West Java possesed morphometric and genetic diversity among populations and lead to assumption that at least there were two cryptic species from M. albus in West Java.

 

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Author Biographies

Lella Herdiana
Sekolah Pascasarjana, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Muhammad Mukhlis Kamal
Departemen Manajemen Sumberdaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Nurlisa Alias Butet
Departemen Manajemen Sumberdaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Ridwan Affandi
Departemen Manajemen Sumberdaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680

References

Abdurahman SW, Ambak MA, Sheriff SM, Mohamed SYAA, Chowdhury AJK. 2016. Morphological variations of plicofollis species (Siluriformes: Ariidae) in Peninsular Malaysia: An insight into truss network approach. Sains Malaysiana. 45(1): 1-7.

Affandi R, Ernawatir Y, Wahyudi S. 2003. Studi bioekologi belut sawah (Monopterus albus) pada berbagai ketinggian tempat di Kabupaten Subang, Jawa Barat. Jurnal Iktiologi Indonesia. 2(3): 49-55.

Allendorf FW. 1988. Conservation biology of fishes. Conservation Biology. 2(2): 145-148. http://doi.org/d7m7bc

Allen US. 2011. Monopterus albus. Invasive species compendium. CAB International, Wallingford Oxon (UK): OX10 8DE.

Atif AB, Zahri MK, Esa AR, Zilfalil BA, Rao USM, Nordin S. 2015. Comparative analysis of the antibacterial, antifungal, antiproliferative and cyclic response element (CRE) induced expression of downstream luc gene activities of Monopterus albus and Channa straitus extracts. Journal of Applied Pharmaceutical Science. 5(1): 42-47.

Arisuryanti T. 2016. Molecular genetic and taxonomic studies of the swamp eel (Monopterus albus Zuiew 1793). [Thesis]. Research Institute for the Environment and Livelihoods Charles. Australia (AU): Darwin University.

[BPS] Badan Pusat Statistika. 2015. Produksi Perikanan Tangkap Indonesia menurut Provinsi dan subsektor (ton), 2000-2015. [Internet] [diunduh 2016 Juli 4]. Tersedia pada https://www.bps.go.id/linkTabelStatis/view/id/1705

Bailey RM, Gans C. 1998. Two new Synbranchid fishes Monopterus roseni from Peninsular India and Monopterus desilvai from Sri Langka. Occasional Papers of the Museum of Zoology The University of Michigan. 726: 1-18.

Britz R, Lalremsanga HT, Lalrotluanga, Lalramliana. 2011. Monopterus ichthyophoides, a new species of scaled swamp eel (Teleostei: Synbranchiformes: Synbranchidae) from Mizoram, India. Zootaxa. 2936: 51-58.

Britz R, Doherty-Bone TM, Kouete MT, Sykes D, Gower DJ. 2016. Monopterus luticolus, a new species of swamp eel from Cameroon (Teleostei: Synbranchidae). Ichthyological Exploration of Freshwaters. 27(4): 309-323.

Chaklader MR, Siddik MAB, Nahar A. 2015. Taxonomic diversity of paradise threadfn Polynemus paradiseus (Linnaeus, 1758) inhabiting southern coastal rivers in Bangladesh. Sains Malaysia. 44(9): 1241-1248. http://doi.org/cgxh

Chen D, Chu W, He Y, Liang XF, Mai K. 2015. Characteristics and phylogenetic studies of complete mitochondrial DNA based on ricefield eel (Monopterus albus) from four different areas. Journal Mitochondrial DNA Part A. 27(4): 2419-2420. http://doi.org/cgxj

Collins MT, Trexler JC, Nico LG, Rawlings TA. 2002. Genetic Diversity in a morphologically conservative invasive taxon: Multiple introductions of swamp eels to the Southeastern United States. Iconservation Biology. 16(4): 1024-1035. http://doi.org/chf8sd

Dahanukar N. 2010. Monopterus cuchia. In: IUCN 2013. IUCN Red List of Threatened Species. Version 2015. 2. [Internet]. [diunduh 23 Agustus 2016]. Tersedia pada: http://www.iucnredlist.org

Dahruddin H, Hutama A, Busson F, Sauri S, Hanner R, Keith P, Hadiaty R, Hubert N. 2016. Revisiting the ichthyodiversity of Java and Bali through DNA barcodes: Taxonomic coverage, identification accuracy, cryptic diversity and identification of exotic species. Molecular Ecology Resources. 17(2): 288-299. http://doi.org/f9x5sx

[DJPB] Ditjen Perikanan Budi Daya. 2012. Belut, komoditas ekspor yang sudah dapat dibudidayakan. [diunduh 2012 Mei 13]. Tersedia pada http://www.kkp.go.id.

Frankham R, Ballou JD, Briscoe DA. 2002. Introduction to conservation genetics. England (UK): Cambridge University Press. http://doi.org/cgxk

Gopi KC. 2002. A new synbranchid fish, Monopterus digressus from Kerala, Peninsular India. Records of the Zoological Survey of India. 100: 137-143.

Hedrick PW. 2001. Conservation genetics: Where are we now?. Trends in Ecology & Evolution. 16(11): 629-636. http://doi.org/dmkxmm

Hellberg ME, Burton RS, Neigel, Palumbi SR. 2002. Genetic assessment of connectivity among marine populations. Bulletin of Marine Science. 70(1): 273-290.

Hossain MAR, Nahiduzzaman M, Saha D, Khanam MUH, Alam MS. 2010. Landmark-based morpho-metric and meristic variations of the endangered carp, Kalibaus Labeo calbasu, from stocks of two isolated rivers, the Jamuna and Halda, and a Hatchery. Zoological Studies. 49(4): 556-563.

Hubert N, Hanner R, Holm E. 2008. Identifying Canadian freshwater fishes through DNA barcodes. Plos One. 3: 1-8. http://doi.org/c6sdhq

Ikram NMMN, Ridzwan BH. 2013. A preliminary screening of antifungal activities from skin mucus extract of Malaysian local swamp eel (Monopterus albus). International Research Journal of Pharmacy and Pharmacology. 3(1): 1-8.

Ho JKI, Ramchunder SJ, Memory A, Theng M, Tianjiao Li. 2016. A guide to the freshwater fauna of nee soon swamp forest. Singapore (SG): Tropical Marine Science Institute, National University of Singapore.

Iskandariah, Soelistyowati DT, Gustiano R, Kusmini II, Huwoyon GH. 2015. Ragam genetik tiga populasi sepat siam (Trichopodus pectoralis Regan; Osphronemidae) asal Kalimantan menggunakan analisis RAPD dan pengukuran morphometric truss. Berita Biologi. 14(1): 57-68.

Kenchington E, Heino M. 2002. Maintenance of genetic diversity: challenges for management of marine resources. International Council for the Exploration of the Sea. Y: 13.

Kottelat M. 2013. The fishes of inland waters of Southeast Asia: A catalogue and core bibliography of fishes known to occur in freshwaters, mangroves and estuaries. The Raffles Bulletin of Zoology. Supplement 27: 1-663.

Kottelat M, Whitten AJ, Kartikasari SN, Wirjoatmojo S. 1993. Freshwater fishes of Western Indonesia and Sulawesi. Hong Kong (HK): Periplus Editions Limited.

Kusmini II, Gustiano R, Radona D, Prakoso VA, Putri FP, Prihadi TH. 2016. Karakterisasi fenotipe dan genotipe tiga populasi ikan tengadak, Barbonymus schwanenfeldii. Jurnal Riset Akuakultur. 11(3): 207-216. http://doi.org/cgxm

Laudien J, Flint NS, Van Der Bank FH, Brey T. 2003. Genetic and morphological variation in four populations of the surf clam Donax serra (Rӧding) from southern African sandy beaches. Biochemical Systematics, and Ecology. 31(7): 751-772. http://doi.org/c9dgd7

Liem KF. 1967. Functional morphology of the integumentary, respiratory, and digestive systems of the synbranchoid fish Monopterus albus. Copeia. 1967(2): 375-388. http://doi.org/b7km24

Mabuchi K, Seno H, Suzuki T, Nishida M. 2005. Discovery of an ancient lineage of Cyprinus carpio from Lake Biwa, Central Japan, based on mtDNA sequence data, with reference to possible multipleorigins of koi. Journal of Fish Biology. 66(6): 1516-1528. http://doi.org/bbq5vk

Matsumoto S, Kon T, Yamaguchi M, Takeshima H, Yamazaki Y, Mukai T, Kuriiwa K, Kohda M, Nishida M. 2010. Cryptic diversification of the swamp eel Monopterus albus in East and Southeast Asia, with special reference to the Ryukyuan populations. Ichthyological Research. 57(1): 71-77. http://doi.org/b8mgsv

Miya M, Kawaguchi A, Nishida M. 2001. Mitogenomic exploration of higher teleostean phylogenies: A case study for moderate-scale evolutionary genomics with 38 newly determined complete mitochondrial DNA sequences. Molecular Biology and Evolution. 18(11): 1993-2009. http://doi.org/fzhtjt

Miah MF, Ali H, Zannath E, Shuvra TM, Naser MN, Ahmed MK. 2015. Breeding biology and induced breeding status of freshwater mud eel, Monopterus cuchia. International Scholarly and Scientific Research & Innovation. 9(6): 633-637.

Mulyasari. 2009. Karakteristik fenotipe morfometrik dan keragaman genotipe RAPD (Random Amplified Polymorphic DNA) ikan nilem (Osteochilus hasselti) di Jawa Barat. [Tesis]. Bogor (ID): Institut Pertanian Bogor.

Mohaddasi M, Shabanipour N, Abdolmaleki S. 2013. Morphometric variation among four populations of Shemaya (Alburnus chalcoides) in the south of Caspian Sea using truss network. The Journal of Basic & Applied Zoology. 66(2): 87-92. http://doi.org/cgxn

Nei M. 1972. Genetic Distance between populations. The American Naturalist.106(949): 283-292. http://doi.org/btq9xj

Nichols JT. 1943. The freshwater fishes of China. New York (US): The American Museum of Natural History.

Osawa S, Su Z-H, Imura Y. 2004. Molecular phylogeny and evolution of carabid graound beetles. Tokyo (JP): Springer Japan. http://doi.org/cpb47x

Palumbi SR. 1994. Genetic Divergence, reproductive isolation, and marine speciation . Annual Review of Ecology, Evolution, and Systematics. 25: 547-572. http://doi.org/cdjh25

Qonita Y. 2015. Keragaman morfometrik kerang gelatik (Anadara pilula Reeve 1843) di Pesisir Pulau Jawa. [Tesis]. Bogor (ID): Institut Pertanian Bogor.

Ratnasingham S, Hebert PDN. 2013. A DNA-based registry for all animal species: the barcode index number (BIN) system. Plos One. 8(8): 66-213. http://doi.org/f4966r

Rosen DE, Greenwood PH. 1976. A fourth neotropical species of synbranchid eel and the phylogeny and systematics of synbranchiform fishes. American Museum of Natural History. 157: 1-70.

Seehausen O, Wagner CE. 2014. Speciation in Freshwater Fishes. Annual Review of Ecology, Evolution, and Systematics. (45): 621-651. http://doi.org/cgxp

Setijaningsih L, Arifin OZ, Gustiano R. 2007. Karakterisasi tiga strain ikan gurami (Osphrone-mus gouramy Lac.) berdasarkan metode truss morfomeriks. Jurnal Ikhtiologi Indonesia. 7(1): 23-30.

Siddik MAB, Hanif MA, Chaklader MR, Nahar A, Fotedar R. 2016. A multivariate morphometric investigation to delineate stock structure of gangetic whiting, Sillaginopsis panijus (Teleostei: Sillaginidae). Springer Plus. 5: 520. http://doi.org/cgxq

Soewardi K. 2007. Pengelolaan keragaman genetik sumber daya perikanan dan kelautan. Departemen Manajemen Sumberdaya Perairan. Bogor (ID): Institut Pertanian Bogor.

Solomon SG, Okomoda VT, Ogbenyikwu AI. 2015. Intraspecific morphological variation between cultured and wild Clarias gariepinus (Burchell) (Clariidae, Siluriformes). Archives of Polish Fisheries. 23(1): 53-61. http://doi.org/cgxr

Swain DP, Riddell B, Murray C. 1991. Morphological differences between hatchery and wild populations of Coho salmon (Oncorhynchus kisutch): Environmental versus genetic origin. Canadian Journal of Fisheries and Aquatic Sciences. 48(9): 1783-1791. http://doi.org/fsgsjd

Tamura K, Stecher G, Peterson D, Kumar S. 2013. MEGA 6: Molecular evolutionary genetics analysis Version 6.0. Journal Molecular Biology and Evolution. 30(12): 2725-2729. http://doi.org/5v5

Turan C, Yalcin S, Turan F, Okur E, Akyurt I. 2005. Morphometric comparisons of African catfish, Clarias gariepinus populations in Turkey. Folia Zoologica. 54: 165-172.

Wijana IMS. 1999. Keragaman enzim dan morfologi belut, Monopterus albus Zuiew (Synabranchidea: Synabarnchidae). [Tesis]. Bogor (ID): Institut Pertanian Bogor.

Wimberger PH. 1992. Plasticity of fish body shape, the effects of diet, development, family and age in two species of Geophagus (Pisces: Cichlidae). Biological Journal of the Linnean Society. 45: 197-218. http://doi.org/bz69r9

Published
2018-01-08
How to Cite
HerdianaL., KamalM. M., ButetN. A., & AffandiR. (2018). Keragaman Morfometrik dan Genetik Gen COI Belut Sawah (Monopterus albus) Asal Empat Populasi di Jawa Barat. Jurnal Ilmu Pertanian Indonesia, 22(3), 180-190. https://doi.org/10.18343/jipi.22.3.180

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