Produksi IgY Spesifik Staphylococcus aureus dari Isolat Asal Kasus Staphylococcosis pada Kelinci
AbstractThis experiment aims to produce immunoglobulin Y (Ig-Y) Staphylococcus aureus isolates origin staphylococcosis case in rabbits. Vaccination is done several times using Staphylococcus aureus active in 109 cfu/ml. Injecting the chicken is done four weeks in a row, the first week is done by intravenous injection of S. aureus antigens, the second week of the injection sub cutan S. aureus emulsified with Freund's complete adjuvant, followed by the third and fourth week of the injection of S. aureus antigen emulsified with incomplete Freund's adjuvant. Egg samples were taken after four weeks of the last injection for identification, purification, and determination of specific IgY against S. aureus in egg yolk. IgY specificity qualitatively tests performed by the AGP test (To Gel Precipitation). IgY extraction is done using PEG - Ammonium sulfate, purified IgY concentration calculated by the method of Bradford. The results showed that IgY began to be detected in egg yolk at week six after immunization, with the average levels of IgY is 1.7 mg/ml.
Abdou AM, Manal MME, Yamashita Y, Kim M. 2014. Immunoglobulin: A Natural Way to Suppress Helicobacter pylori in Humans. Health. 6: 781-791. http://doi.org/b4pk
Bien J, Sokolova O, Bozko P. 2011. Characterization of Virulence Factors of Staphylococcus aureus: Novel Function of Known Virulence Factors That Are Implicated in Activation of Airway Epithelial Proinflammatory Response. Journal of Pathogens. 2011. Article ID 601905. 13 pages. http://doi.org/djr3w6
Dai YC, Zhang XF, Tan M, Huang P, Lei W, Fang H, Zhong W, Jiang X. 2013. A dual chicken IgY against rotavirus and norovirus. Antiviral Research. 97(3): 293-300. http://doi.org/f4ttz5
Diraviyam T, Zhao B, Wang Y, Schade R, Michael A, Zhang X. 2014. Effect of Chicken Egg Yolk Antibodies (IgY) against Diarrhea in Domesticated Animals: A Systematic Review and Meta-Analysis. PLoS One. 9(5): e97716. http://doi.org/b2qm
de Paula VS, da Silva AS, de Vasconcelos GA, Iff ET, Silva ME, Kappel LA. 2011. Applied biotechnology for production of immunoglobulin Y specific to hepatitis A virus. Journal of Virological Methods. 171(1): 102-106. http://doi.org/cw52n7
Farooq A, Rabbani M, Muhammad K, Akram Z, Ahad A, Fatima Z, Kamal T, Anwar Z. 2012. Passive immunization in infectious bursal disease virus infected birds using chemically purified immune yolk immunoglobulins (IgY). African Journal of Microbiology Research. 6(12): 2993-2998. http://doi.org/b2qn
Ferreira A, Monteiro JM, Vieira-Pinto M. 2014. The importance of subcutaneous abscess infection by Pasteurella spp. And Staphylococcus aureus as a cause of meat condemnation in slaughtered commercial rabbits. World Rabbit Science. 22(4): 311-317. http://doi.org/b2qp
Hermans D, Steendam K, Verbrugghe E, Verlinden M, Martel A, Seliwiorstow T, Heyndrick M, Haesebrouck F, Zutter L, Deforce D, Pasman F. 2014. Passive immunization to reduce Campylo-bacter jejuni colonization and transmission in broiler chicken. Veterinary Research. 2014. 45: 27. http://doi.org/b2qq
Hiroko SP, Kurtini T, Riyanti. 2014. Pengaruh lama simpan dan warna kerabang telur ayam ras terhadap indeks albumin, indeks yolk dan pH telur. Jurnal Ilmiah Peternakan Terpadu. 2(3): 108-114.
Indrawati A. 2010. Telur Ayam 3 in One Anti Flu Burung dan Anti Diare: Produksi, Efikasi dan Aplikasinya. [Laporan Akhir Penelitian]. Bogor (ID): Institut Pertanian Bogor.
Kalenik B, Sawicka R, Góra-Sochacka A, Sirko A. 2014. Influenza prevention and treatment by passive immunization. Acta Biochimica Polonica. 61(3): 573-587.
Kovacs-Nolan J, Mine Y. 2012. Egg yolk antibodies for passive immunity. Annual Review of Food Science and Technology. 3: 163-182. http://doi.org/b2qr
Lin YF, Liou JF, Chen LR, Lien TF. 2014. IgY Production by Taiwan Native Tsaiya Ducks and White Leghorn Laying Hens. Journal of Agricultural Science. 6(9): 106-112. http://doi.org/b2qs
Matheis W, Schade R. 2011. Development of an IgY-based rocket-immunoelectrophoresis for identity monitoring of Pertussis vaccines. Journal of Immunological Methods. 369(1-2): 125-132. http://doi.org/b85sqv
Mahde NK. 2011. The pathogenesis of experimental infection by Staphylococcus aureus in rabbits. Kufa Journal for Veterinary Medical Sciences. 2(2): 127-140.
Pauly D, Chacana PA, Calzado EG, Brembs B, Schade R. 2011. IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation. Journal of Visualized Experiments. 51: 167-174. http://doi.org/ccvjm2
Prabhudas M, Adkins B, Gans H. 2011. Challenges in infant immunity: implications for responses to infection and vaccines. Nature Immunology. 12: 189-194. http://doi.org/fjfvqk
Priyanka BS, Abhijith KS, Navin KR, Raghavarao MS, Thakur MS. 2014. Integrated Approach for the Extraction and Purification of IgY from Chicken Egg Yolk. Separation Science and Technology. 49(4): 562-568. http://doi.org/b2qt
Sentila R, Karthika S, Michael A, Gandhimathi A. 2013. Protection against Dental Carries by Passive Immunization with Hen Egg Yolk Antibody Using Cell Associated Glucosyltransferase of Streptococcus mutans. Journal of Medical Microbiology & Diagnosis. 2(3): 1-5. http://doi.org/b2qv
Tan SH, Mohamedali A, Kapur A, Lukjanenko L, Baker MS. 2012. A novel, cost-effective and efficient chicken egg IgY purification procedure. Journal of Immunological Methods. 380(1-2): 73-76. http://doi.org/b2qw
Tobias FL, Garcia LNN, Kanashiro MM, Acosta EM, João G, Vieira-da-Motta O. 2012. Growth Inhibition of Staphylococcus aureus and Escherichia coli Strains by Neutralizing IgY Antibodies From Ostrich Egg Yolk. Brazilian Journal of Microbiology. 43(2): 544-551. http://doi.org/b2qx
Wang B, Yang J, Cao S, Wang H, Pan X, Zhu J, Zhou Y, Gao L, Li W, Li M. 2014. Preparation of specific anti-Helicobacter pylori yolk antibodies and their antibacterial effects. International Journal of Clinical & Experimental Medicine. 7(10): 6430-6437.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).