Induksi Kalus Jeruk Kasturi (Citrus microcarpa) Menggunakan Hormon 2,4-D dan BAP dengan Metode in vitro

Imam Mahadi, Wan Syafi’i, Yeni Sari

Abstract


Calamansi fruits (Citrus microcarpa) or musk lime is one horticulture crops has been known as beverage and food flavorings. This research aimed to determine the effect of 2,4-D and BAP on callus induction of explants calamansi fruits (Citrus microcarpa). Parameters observed is percentage of explants, time appeared callus, callus width, and texture of the callus. Research results and a growing percentage of the width callus explants were analyzed by ANOVA and a further test DMRT at 5% level. The next to appear callus timing parameters and texture of callus analyzed in description. The result shows that a combination of hormones D4B1 and D4B2 produces a callus emerged fastest of 3.3 days after culture. The highest percentage of callus formation that is 100% contained in the treatment of D2B0, D2B0.5-D4B0. The mean width of callus was highest in treatment D4B2with a width of 0.97 cm. Embryogenic callus resulting by treatment of D2B1, D2B2, and D3B0. However, the best a combination of hormones is treatment D2B2 (2 mg/l, 2,4-D, dan 2 mg/l BAP) producing embryogenic callus edvanced to suspension cultures.

Keywords


BAP, callus embryogenic, explant calamansi, hormone 2,4-D

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References


Abidin Z. 1982. Dasar-dasar Pengetahuan Tentang Zat Pengatur Tumbuh. Bandung (ID): Angkasa.

Ardiana DW. A. 2009. Tekhnik Pemberian Benzyl Amino Purin untuk Memacu Pertumbuhan Kalus dan Tunas pada Kotiledon Melon (Cucumis melo L.). Buletin Tekhnik Pertanian. 14(2): 50-53.

Balilashaki K, Vahedi M, Karimi R. 2015. In vitro direct regeneration from node and leaf explant of Phalaenopsis cv. Surabaya. Plant Tissue Culture & Bitechnology. 25(2): 193-205. http://doi.org/bpqd

Bal JS. 1997. Fruit Growing. New Delhi (IN): Kalyani Publishers.

Bienaime C, Melin A, Bensaddek L, Attoumbre J, Nava-Saucedo E, Baltora-Rosset S. 2015. Effects of plant growth regulators on cell cultures of Lycopodiella inundata. Plant Cell, Tissue and Organ Culture. 123(3): 523-533. http://doi.org/bpqf

Govinden-Soulange J, Boodia N, Dussooa C, Gunowa R, Deensah S, Facknath S, Rajkomar B. 2009. Vegetative Propagation and Tissue Culture of Hibiscus sabdariffa L. (Roselle). World Journal of Agricultural Sciences. 5(5): 651-661.

Gunawan LW. 1992. Tekhnik Kultur Jaringan Tumbuhan. Bogor (ID): Pusat Antar Universitas. Bioteknologi. Institut Pertanian Bogor.

Hartman HT, Kester DE. 1983. Plant Propagation Principles and Practice. New Jersey (US): Prentice Hall, Inc.

Mahadi I. 2012. Induksi Kalus Kenerak (Goniothalamus umbrosus) berdasarkan jenis eksplan menggunakan metode In Vitro. Jurnal Agroteknologi Tropika. 1(1): 18-22.

Mahadi I, Wulandari S, Omar A. 2014. Pengaruh Naftalen Acetyl Acid (NAA) dan Benzyl Amino Purin (BAP) Terhadap Pembentukan Kalus Tanaman Rosella (Hibiscus Sabdariffa) sebagai Sumber Belajar Konsep Bioteknologi Bagi Siswa SMA. Jurnal Biogenesis. 11(1): 1-7.

Kristina NN. 2008. Multiplikasi Tunas dan aklimatisasi Pegagan (Centella asiatica) periode kultur lima tahun. Jurnal Littri. 14(1): 30-35.

Peterson G, Smith R. l991. Effect of abscicic acid and callus size onregeneration of American and international rice varieties. Plant Cell Reports. 10(1): 35-38. http://doi.org/fr6ng9

Rahmi I, Suliansyah I, Bustamam T. 2010. Pengaruh Pemberian Beberapa Konsentrasi BAP dan NAA Terhadap Multiplikasi Tunas Pucuk Jeruk Kanci (Citrus sp.) Secara In Vitro. Jerami. 3(3): 210-219.

Robles-Martinez M, Barba-de la Rosa AP, Gueroud F, Negre-Salvayre A, Rossognol M, Santos-Diaz MS. 2016. Establishment of callus and cell suspensions of wild and domesticated Opuntia Species: Study on their potential as a source of metabolite production. Plant Cell, Tissue and Organ Culture. 124(1): 181-189. http://doi.org/bpqg

Samudin S. 2009. Pengaruh Kombinasi Auksin-Sitokinin Terhadap Pertumbuhan Buah Naga. Media Litbang Sulteng. 2(1): 62-66.

Wardani DP, Solichatun, Setyawan AD. 2004. Pertumbuhan dan Produksi Saponin Kultur Kalus Talinum paniculatum Gaertn Pada Variasi Penambahan Asam 2,4-Diklorofenoksi Asetat (2,4-D) dan Kinetin. Biofarmasi. 2(1): 35-43.

Yusnita. 2003. Kultur Jaringan Cara memperbanyak Tanaman secara Efisien. Jakarta (ID): Agromedia Pustaka.

Zulkarnain. 2009. Kultur Jaringan TanamanSolusi Perbanyakan Tanaman Budi daya. Jakarta (ID): Bumi Aksara.




DOI: https://doi.org/10.18343/jipi.21.2.84

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